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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Burkholderia xenovorans DNA repair protein recO (recO) CSB-YP622739BNV
CSB-EP622739BNV
CSB-BP622739BNV
CSB-MP622739BNV
CSB-EP622739BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Phosphoglucosamine mutase (glmM) CSB-YP622740BNV
CSB-EP622740BNV
CSB-BP622740BNV
CSB-MP622740BNV
CSB-EP622740BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Lysine--tRNA ligase (lysS), partial CSB-YP622741BNV
CSB-EP622741BNV
CSB-BP622741BNV
CSB-MP622741BNV
CSB-EP622741BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP622742BNV
CSB-EP622742BNV
CSB-BP622742BNV
CSB-MP622742BNV
CSB-EP622742BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Probable potassium transport system protein kup (kup), partial CSB-YP622743BNV
CSB-EP622743BNV
CSB-BP622743BNV
CSB-MP622743BNV
CSB-EP622743BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Glutamate--tRNA ligase (gltX) CSB-YP622744BNV
CSB-EP622744BNV
CSB-BP622744BNV
CSB-MP622744BNV
CSB-EP622744BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Elongation factor Ts (tsf) CSB-YP622745BNV
CSB-EP622745BNV
CSB-BP622745BNV
CSB-MP622745BNV
CSB-EP622745BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Protein RnfH (rnfH) CSB-YP622746BNV
CSB-EP622746BNV
CSB-BP622746BNV
CSB-MP622746BNV
CSB-EP622746BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Putative reductase Bxeno_A2233 (Bxeno_A2233) CSB-YP622747BNV
CSB-EP622747BNV
CSB-BP622747BNV
CSB-MP622747BNV
CSB-EP622747BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP622748BNV
CSB-EP622748BNV
CSB-BP622748BNV
CSB-MP622748BNV
CSB-EP622748BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans UPF0133 protein Bxeno_A2072 (Bxeno_A2072) CSB-YP622749BNV
CSB-EP622749BNV
CSB-BP622749BNV
CSB-MP622749BNV
CSB-EP622749BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans 60 kDa chaperonin 2 (groL2), partial CSB-YP622750BNV
CSB-EP622750BNV
CSB-BP622750BNV
CSB-MP622750BNV
CSB-EP622750BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Coactosin-like protein (COTL1) CSB-YP622751HU
CSB-BP622751HU
CSB-MP622751HU
CSB-EP622751HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Desmoglein-2 (DSG2), partial CSB-YP622752HU
CSB-EP622752HU
CSB-EP622752HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human MORC family CW-type zinc finger protein 3 (MORC3), partial CSB-YP622753HU
CSB-BP622753HU
CSB-MP622753HU
CSB-EP622753HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ubiquitin-associated protein 2-like (UBAP2L), partial CSB-YP622754HU
CSB-EP622754HU
CSB-BP622754HU
CSB-MP622754HU
CSB-EP622754HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Tubulin--tyrosine ligase-like protein 12 (TTLL12) CSB-YP622755HU
CSB-EP622755HU
CSB-BP622755HU
CSB-MP622755HU
CSB-EP622755HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Dihydropyrimidinase-related protein 3 (DPYSL3) CSB-YP622756HU
CSB-EP622756HU
CSB-BP622756HU
CSB-MP622756HU
CSB-EP622756HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans N-succinylglutamate 5-semialdehyde dehydrogenase (astD) CSB-YP622757BNV
CSB-EP622757BNV
CSB-BP622757BNV
CSB-MP622757BNV
CSB-EP622757BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein BTG3 (BTG3) CSB-YP622758HU
CSB-EP622758HU
CSB-BP622758HU
CSB-MP622758HU
CSB-EP622758HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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