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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia xenovorans Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP623878BNV
CSB-EP623878BNV
CSB-BP623878BNV
CSB-MP623878BNV
CSB-EP623878BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB) CSB-YP623879BNV
CSB-EP623879BNV
CSB-BP623879BNV
CSB-MP623879BNV
CSB-EP623879BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP623880BNV
CSB-EP623880BNV
CSB-BP623880BNV
CSB-MP623880BNV
CSB-EP623880BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans UPF0434 protein Bxeno_A3631 (Bxeno_A3631) CSB-YP623881BNV
CSB-EP623881BNV
CSB-BP623881BNV
CSB-MP623881BNV
CSB-EP623881BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP623882BNV
CSB-EP623882BNV
CSB-BP623882BNV
CSB-MP623882BNV
CSB-EP623882BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Fructose-1,6-bisphosphatase class 1 1 (fbp1) CSB-YP623883BNV
CSB-EP623883BNV
CSB-BP623883BNV
CSB-MP623883BNV
CSB-EP623883BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Integration host factor subunit beta (ihfB) CSB-YP623884BNV
CSB-EP623884BNV
CSB-BP623884BNV
CSB-MP623884BNV
CSB-EP623884BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Maf-like protein Bxeno_A3340 (Bxeno_A3340) CSB-YP623885BNV
CSB-EP623885BNV
CSB-BP623885BNV
CSB-MP623885BNV
CSB-EP623885BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Chorismate synthase (aroC) CSB-YP623886BNV
CSB-EP623886BNV
CSB-BP623886BNV
CSB-MP623886BNV
CSB-EP623886BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Lipoprotein-releasing system ATP-binding protein LolD (lolD) CSB-YP623887BNV
CSB-EP623887BNV
CSB-BP623887BNV
CSB-MP623887BNV
CSB-EP623887BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans GTPase Der (der) CSB-YP623888BNV
CSB-EP623888BNV
CSB-BP623888BNV
CSB-MP623888BNV
CSB-EP623888BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Cysteine--tRNA ligase (cysS) CSB-YP623889BNV
CSB-EP623889BNV
CSB-BP623889BNV
CSB-MP623889BNV
CSB-EP623889BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP623890BNV
CSB-EP623890BNV
CSB-BP623890BNV
CSB-MP623890BNV
CSB-EP623890BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans SsrA-binding protein (smpB) CSB-YP623891BNV
CSB-EP623891BNV
CSB-BP623891BNV
CSB-MP623891BNV
CSB-EP623891BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Thymidylate kinase (tmk) CSB-YP623892BNV
CSB-EP623892BNV
CSB-BP623892BNV
CSB-MP623892BNV
CSB-EP623892BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Trigger factor (tig) CSB-YP623893BNV
CSB-EP623893BNV
CSB-BP623893BNV
CSB-MP623893BNV
CSB-EP623893BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans 23S rRNA (uracil (1939)-C (5))-methyltransferase RlmD (rlmD) CSB-YP623894BNV
CSB-EP623894BNV
CSB-BP623894BNV
CSB-MP623894BNV
CSB-EP623894BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans 30S ribosomal protein S6 (rpsF) CSB-YP623895BNV
CSB-EP623895BNV
CSB-BP623895BNV
CSB-MP623895BNV
CSB-EP623895BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Ribose-5-phosphate isomerase A (rpiA) CSB-YP623896BNV
CSB-EP623896BNV
CSB-BP623896BNV
CSB-MP623896BNV
CSB-EP623896BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Cyclic nucleotide-gated cation channel beta-1 (CNGB1), partial CSB-YP623897HU
CSB-BP623897HU
CSB-MP623897HU
CSB-EP623897HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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