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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Burkholderia xenovorans Periplasmic nitrate reductase (napA), partial CSB-YP623858BNV
CSB-EP623858BNV
CSB-BP623858BNV
CSB-MP623858BNV
CSB-EP623858BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Acylphosphatase (acyP) CSB-YP623859BNV
CSB-EP623859BNV
CSB-BP623859BNV
CSB-MP623859BNV
CSB-EP623859BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Putative cobalt-precorrin-6A synthase [deacetylating] (cbiD) CSB-YP623860BNV
CSB-EP623860BNV
CSB-BP623860BNV
CSB-MP623860BNV
CSB-EP623860BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans 2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase (mhpB) CSB-YP623861BNV
CSB-EP623861BNV
CSB-BP623861BNV
CSB-MP623861BNV
CSB-EP623861BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Selenide, water dikinase (selD) CSB-YP623862BNV
CSB-EP623862BNV
CSB-BP623862BNV
CSB-MP623862BNV
CSB-EP623862BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Membrane protein insertase YidC (yidC), partial CSB-YP623863BNV1
CSB-EP623863BNV1
CSB-BP623863BNV1
CSB-MP623863BNV1
CSB-EP623863BNV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans ATP synthase subunit alpha 2 (atpA2), partial CSB-YP623864BNV
CSB-EP623864BNV
CSB-BP623864BNV
CSB-MP623864BNV
CSB-EP623864BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans 30S ribosomal protein S21 1 (rpsU1) CSB-YP623865BNV
CSB-EP623865BNV
CSB-BP623865BNV
CSB-MP623865BNV
CSB-EP623865BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans 50S ribosomal protein L1 (rplA) CSB-YP623866BNV
CSB-EP623866BNV
CSB-BP623866BNV
CSB-MP623866BNV
CSB-EP623866BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans 30S ribosomal protein S7 (rpsG) CSB-YP623867BNV
CSB-EP623867BNV
CSB-BP623867BNV
CSB-MP623867BNV
CSB-EP623867BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans 50S ribosomal protein L23 (rplW) CSB-YP623868BNV
CSB-EP623868BNV
CSB-BP623868BNV
CSB-MP623868BNV
CSB-EP623868BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans 50S ribosomal protein L14 (rplN) CSB-YP623869BNV
CSB-EP623869BNV
CSB-BP623869BNV
CSB-MP623869BNV
CSB-EP623869BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans 30S ribosomal protein S5 (rpsE) CSB-YP623870BNV
CSB-EP623870BNV
CSB-BP623870BNV
CSB-MP623870BNV
CSB-EP623870BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans 30S ribosomal protein S4 (rpsD) CSB-YP623871BNV
CSB-EP623871BNV
CSB-BP623871BNV
CSB-MP623871BNV
CSB-EP623871BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Protein CyaY (cyaY) CSB-YP623872BNV
CSB-EP623872BNV
CSB-BP623872BNV
CSB-MP623872BNV
CSB-EP623872BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP623873BNV
CSB-EP623873BNV
CSB-BP623873BNV
CSB-MP623873BNV
CSB-EP623873BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans 2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (coq7) CSB-YP623874BNV
CSB-EP623874BNV
CSB-BP623874BNV
CSB-MP623874BNV
CSB-EP623874BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP623875BNV
CSB-EP623875BNV
CSB-BP623875BNV
CSB-MP623875BNV
CSB-EP623875BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP623876BNV
CSB-EP623876BNV
CSB-BP623876BNV
CSB-MP623876BNV
CSB-EP623876BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Proline--tRNA ligase (proS), partial CSB-YP623877BNV
CSB-EP623877BNV
CSB-BP623877BNV
CSB-MP623877BNV
CSB-EP623877BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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