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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shigella boydii serotype 4 UPF0253 protein yaeP (yaeP) CSB-YP653344SGE
CSB-EP653344SGE
CSB-BP653344SGE
CSB-MP653344SGE
CSB-EP653344SGE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 4 Outer membrane protein assembly factor YaeT (yaeT), partial CSB-YP653345SGE
CSB-EP653345SGE
CSB-BP653345SGE
CSB-MP653345SGE
CSB-EP653345SGE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 4 Deoxyguanosinetriphosphate triphosphohydrolase (dgt), partial CSB-YP653346SGE
CSB-EP653346SGE
CSB-BP653346SGE
CSB-MP653346SGE
CSB-EP653346SGE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marchantia polymorpha Uncharacterized 6.4 kDa protein in atpA-psbA intergenic region, partial CSB-YP653347MCB
CSB-EP653347MCB
CSB-BP653347MCB
CSB-MP653347MCB
CSB-EP653347MCB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nolana spathulata Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP653348NAAQ
CSB-EP653348NAAQ
CSB-BP653348NAAQ
CSB-MP653348NAAQ
CSB-EP653348NAAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 4 UPF0289 protein yacF (yacF) CSB-YP653349SGE
CSB-EP653349SGE
CSB-BP653349SGE
CSB-MP653349SGE
CSB-EP653349SGE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 4 Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP653350SGE
CSB-EP653350SGE
CSB-BP653350SGE
CSB-MP653350SGE
CSB-EP653350SGE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 4 L-arabinose isomerase (araA) CSB-YP653351SGE
CSB-EP653351SGE
CSB-BP653351SGE
CSB-MP653351SGE
CSB-EP653351SGE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 4 Glutathione-regulated potassium-efflux system protein kefC (kefC), partial CSB-YP653352SGE
CSB-EP653352SGE
CSB-BP653352SGE
CSB-MP653352SGE
CSB-EP653352SGE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 4 Na (+)/H (+) antiporter nhaA (nhaA), partial CSB-YP653353SGE
CSB-EP653353SGE
CSB-BP653353SGE
CSB-MP653353SGE
CSB-EP653353SGE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 E3 ubiquitin-protein ligase ipaH9.8 (ipaH9.8), partial CSB-YP653354SGF
CSB-EP653354SGF
CSB-BP653354SGF
CSB-MP653354SGF
CSB-EP653354SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Phosphothreonine lyase ospF (ospF) CSB-YP653355SGF
CSB-EP653355SGF
CSB-BP653355SGF
CSB-MP653355SGF
CSB-EP653355SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Peptide chain release factor 3 (prfC), partial CSB-YP653356SGF
CSB-EP653356SGF
CSB-BP653356SGF
CSB-MP653356SGF
CSB-EP653356SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Glycerol-3-phosphate acyltransferase (plsB), partial CSB-YP653357SGF
CSB-EP653357SGF
CSB-BP653357SGF
CSB-MP653357SGF
CSB-EP653357SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 10 kDa chaperonin (groS) CSB-YP653358SGF
CSB-EP653358SGF
CSB-BP653358SGF
CSB-MP653358SGF
CSB-EP653358SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Adenylosuccinate synthetase (purA) CSB-YP653359SGF
CSB-EP653359SGF
CSB-BP653359SGF
CSB-MP653359SGF
CSB-EP653359SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Primosomal replication protein n (priB) CSB-YP653360SGF
CSB-EP653360SGF
CSB-BP653360SGF
CSB-MP653360SGF
CSB-EP653360SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 HTH-type transcriptional regulator UlaR (ulaR) CSB-YP653361SGF
CSB-EP653361SGF
CSB-BP653361SGF
CSB-MP653361SGF
CSB-EP653361SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Probable cytosol aminopeptidase (pepA), partial CSB-YP653362SGF
CSB-EP653362SGF
CSB-BP653362SGF
CSB-MP653362SGF
CSB-EP653362SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Chlorophyll a-b binding protein 3, chloroplastic (lhca3), partial CSB-YP653363EWE
CSB-EP653363EWE
CSB-BP653363EWE
CSB-MP653363EWE
CSB-EP653363EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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