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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Shigella dysenteriae serotype 1 Chromosomal replication initiator protein DnaA (dnaA) CSB-YP653364SGF
CSB-EP653364SGF
CSB-BP653364SGF
CSB-MP653364SGF
CSB-EP653364SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Uncharacterized protein yjdP (yjdP) CSB-YP653365SGF
CSB-EP653365SGF
CSB-BP653365SGF
CSB-MP653365SGF
CSB-EP653365SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Ribonuclease PH (rph) CSB-YP653366SGF
CSB-EP653366SGF
CSB-BP653366SGF
CSB-MP653366SGF
CSB-EP653366SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP653367SGF
CSB-EP653367SGF
CSB-BP653367SGF
CSB-MP653367SGF
CSB-EP653367SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 ATP synthase subunit beta (atpD) CSB-YP653368SGF
CSB-EP653368SGF
CSB-BP653368SGF
CSB-MP653368SGF
CSB-EP653368SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Protein viaA (viaA) CSB-YP653369SGF
CSB-EP653369SGF
CSB-BP653369SGF
CSB-MP653369SGF
CSB-EP653369SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 4-alpha-L-fucosyltransferase (wecF) CSB-YP653370SGF
CSB-EP653370SGF
CSB-BP653370SGF
CSB-MP653370SGF
CSB-EP653370SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Magnesium transport protein CorA (corA), partial CSB-YP653371SGF1
CSB-EP653371SGF1
CSB-BP653371SGF1
CSB-MP653371SGF1
CSB-EP653371SGF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Xaa-Pro dipeptidase (pepQ) CSB-YP653372SGF
CSB-EP653372SGF
CSB-BP653372SGF
CSB-MP653372SGF
CSB-EP653372SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 HTH-type transcriptional activator rhaS (rhaS) CSB-YP653373SGF
CSB-EP653373SGF
CSB-BP653373SGF
CSB-MP653373SGF
CSB-EP653373SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 ATP-dependent protease subunit HslV (hslV) CSB-YP653374SGF
CSB-EP653374SGF
CSB-BP653374SGF
CSB-MP653374SGF
CSB-EP653374SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP653375SGF
CSB-EP653375SGF
CSB-BP653375SGF
CSB-MP653375SGF
CSB-EP653375SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Phosphomethylpyrimidine synthase (thiC), partial CSB-YP653376SGF
CSB-EP653376SGF
CSB-BP653376SGF
CSB-MP653376SGF
CSB-EP653376SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 3-dehydroquinate synthase (aroB) CSB-YP653377SGF
CSB-EP653377SGF
CSB-BP653377SGF
CSB-MP653377SGF
CSB-EP653377SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Thiosulfate sulfurtransferase glpE (glpE) CSB-YP653378SGF
CSB-EP653378SGF
CSB-BP653378SGF
CSB-MP653378SGF
CSB-EP653378SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 sn-glycerol-3-phosphate transport system permease protein ugpE (ugpE), partial CSB-YP653379SGF1
CSB-EP653379SGF1
CSB-BP653379SGF1
CSB-MP653379SGF1
CSB-EP653379SGF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP653380SGF
CSB-EP653380SGF
CSB-BP653380SGF
CSB-MP653380SGF
CSB-EP653380SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Protein TusC (tusC) CSB-YP653381SGF
CSB-EP653381SGF
CSB-BP653381SGF
CSB-MP653381SGF
CSB-EP653381SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 50S ribosomal protein L23 (rplW) CSB-YP653382SGF
CSB-EP653382SGF
CSB-BP653382SGF
CSB-MP653382SGF
CSB-EP653382SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 50S ribosomal protein L24 (rplX) CSB-YP653383SGF
CSB-EP653383SGF
CSB-BP653383SGF
CSB-MP653383SGF
CSB-EP653383SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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