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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shigella dysenteriae serotype 1 Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP656168SGF
CSB-EP656168SGF
CSB-BP656168SGF
CSB-MP656168SGF
CSB-EP656168SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Phosphate import ATP-binding protein PstB (pstB) CSB-YP656169SGF
CSB-EP656169SGF
CSB-BP656169SGF
CSB-MP656169SGF
CSB-EP656169SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 ATP synthase subunit a (atpB), partial CSB-YP656170SGF1
CSB-EP656170SGF1
CSB-BP656170SGF1
CSB-MP656170SGF1
CSB-EP656170SGF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Dihydroxy-acid dehydratase (ilvD), partial CSB-YP656171SGF
CSB-EP656171SGF
CSB-BP656171SGF
CSB-MP656171SGF
CSB-EP656171SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Tyrosine recombinase XerC (xerC) CSB-YP656172SGF
CSB-EP656172SGF
CSB-BP656172SGF
CSB-MP656172SGF
CSB-EP656172SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Protein FdhE (fdhE) CSB-YP656173SGF
CSB-EP656173SGF
CSB-BP656173SGF
CSB-MP656173SGF
CSB-EP656173SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 6-phosphofructokinase (pfkA) CSB-YP656174SGF
CSB-EP656174SGF
CSB-BP656174SGF
CSB-MP656174SGF
CSB-EP656174SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP656175SGF
CSB-EP656175SGF
CSB-BP656175SGF
CSB-MP656175SGF
CSB-EP656175SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 HTH-type transcriptional repressor fabR (fabR) CSB-YP656176SGF
CSB-EP656176SGF
CSB-BP656176SGF
CSB-MP656176SGF
CSB-EP656176SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Thiazole synthase (thiG) CSB-YP656177SGF
CSB-EP656177SGF
CSB-BP656177SGF
CSB-MP656177SGF
CSB-EP656177SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Ferrous iron transport protein C (feoC) CSB-YP656178SGF
CSB-EP656178SGF
CSB-BP656178SGF
CSB-MP656178SGF
CSB-EP656178SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Nickel-responsive regulator (nikR) CSB-YP656179SGF
CSB-EP656179SGF
CSB-BP656179SGF
CSB-MP656179SGF
CSB-EP656179SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Glycogen debranching enzyme (glgX), partial CSB-YP656180SGF
CSB-EP656180SGF
CSB-BP656180SGF
CSB-MP656180SGF
CSB-EP656180SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Protein tsgA (tsgA), partial CSB-YP656181SGF
CSB-EP656181SGF
CSB-BP656181SGF
CSB-MP656181SGF
CSB-EP656181SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 30S ribosomal protein S7 (rpsG) CSB-YP656182SGF
CSB-EP656182SGF
CSB-BP656182SGF
CSB-MP656182SGF
CSB-EP656182SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 30S ribosomal protein S19 (rpsS) CSB-YP656183SGF
CSB-EP656183SGF
CSB-BP656183SGF
CSB-MP656183SGF
CSB-EP656183SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 50S ribosomal protein L5 (rplE) CSB-YP656184SGF
CSB-EP656184SGF
CSB-BP656184SGF
CSB-MP656184SGF
CSB-EP656184SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 50S ribosomal protein L15 (rplO) CSB-YP656185SGF
CSB-EP656185SGF
CSB-BP656185SGF
CSB-MP656185SGF
CSB-EP656185SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Shikimate dehydrogenase (aroE) CSB-YP656186SGF
CSB-EP656186SGF
CSB-BP656186SGF
CSB-MP656186SGF
CSB-EP656186SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Malate dehydrogenase (mdh) CSB-YP656187SGF
CSB-EP656187SGF
CSB-BP656187SGF
CSB-MP656187SGF
CSB-EP656187SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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