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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shigella dysenteriae serotype 1 D-amino acid dehydrogenase small subunit (dadA) CSB-YP656228SGF
CSB-EP656228SGF
CSB-BP656228SGF
CSB-MP656228SGF
CSB-EP656228SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Flagellar transcriptional regulator FlhD (flhD) CSB-YP656229SGF
CSB-EP656229SGF
CSB-BP656229SGF
CSB-MP656229SGF
CSB-EP656229SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP656230SGF
CSB-EP656230SGF
CSB-BP656230SGF
CSB-MP656230SGF
CSB-EP656230SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Protein dsrB (dsrB) CSB-YP656231SGF
CSB-EP656231SGF
CSB-BP656231SGF
CSB-MP656231SGF
CSB-EP656231SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Probable phosphatase YcdX (ycdX) CSB-YP656232SGF
CSB-EP656232SGF
CSB-BP656232SGF
CSB-MP656232SGF
CSB-EP656232SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Chaperone protein TorD (torD) CSB-YP656233SGF
CSB-EP656233SGF
CSB-BP656233SGF
CSB-MP656233SGF
CSB-EP656233SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Cell division protein ZapC (zapC) CSB-YP656234SGF
CSB-EP656234SGF
CSB-BP656234SGF
CSB-MP656234SGF
CSB-EP656234SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Periplasmic nitrate reductase (napA), partial CSB-YP656235SGF
CSB-EP656235SGF
CSB-BP656235SGF
CSB-MP656235SGF
CSB-EP656235SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 UPF0194 membrane protein ybhG (ybhG) CSB-YP656236SGF
CSB-EP656236SGF
CSB-BP656236SGF
CSB-MP656236SGF
CSB-EP656236SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Putative transport protein YbjL (ybjL), partial CSB-YP656237SGF
CSB-EP656237SGF
CSB-BP656237SGF
CSB-MP656237SGF
CSB-EP656237SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Methylaspartate mutase S chain (mamA) CSB-YP656238SGF
CSB-EP656238SGF
CSB-BP656238SGF
CSB-MP656238SGF
CSB-EP656238SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB) CSB-YP656239SGF
CSB-EP656239SGF
CSB-BP656239SGF
CSB-MP656239SGF
CSB-EP656239SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Protein CrcB homolog (crcB), partial CSB-YP656240SGF1
CSB-EP656240SGF1
CSB-BP656240SGF1
CSB-MP656240SGF1
CSB-EP656240SGF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Xanthine phosphoribosyltransferase (gpt) CSB-YP656241SGF
CSB-EP656241SGF
CSB-BP656241SGF
CSB-MP656241SGF
CSB-EP656241SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Adenylate kinase (adk) CSB-YP656242SGF
CSB-EP656242SGF
CSB-BP656242SGF
CSB-MP656242SGF
CSB-EP656242SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Shikimate kinase 2 (aroL) CSB-YP656243SGF
CSB-EP656243SGF
CSB-BP656243SGF
CSB-MP656243SGF
CSB-EP656243SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 N utilization substance protein B homolog (nusB) CSB-YP656244SGF
CSB-EP656244SGF
CSB-BP656244SGF
CSB-MP656244SGF
CSB-EP656244SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP656245SGF
CSB-EP656245SGF
CSB-BP656245SGF
CSB-MP656245SGF
CSB-EP656245SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Ribonuclease H (rnhA) CSB-YP656246SGF
CSB-EP656246SGF
CSB-BP656246SGF
CSB-MP656246SGF
CSB-EP656246SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Lipid-A-disaccharide synthase (lpxB) CSB-YP656247SGF
CSB-EP656247SGF
CSB-BP656247SGF
CSB-MP656247SGF
CSB-EP656247SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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