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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Listeria monocytogenes serovar 1/2a Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP838122LPY
CSB-EP838122LPY
CSB-BP838122LPY
CSB-MP838122LPY
CSB-EP838122LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Heme A synthase (ctaA), partial CSB-YP838123LPY
CSB-EP838123LPY
CSB-BP838123LPY
CSB-MP838123LPY
CSB-EP838123LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP838124LPY
CSB-EP838124LPY
CSB-BP838124LPY
CSB-MP838124LPY
CSB-EP838124LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a L-aspartate oxidase (nadB) CSB-YP838125LPY
CSB-EP838125LPY
CSB-BP838125LPY
CSB-MP838125LPY
CSB-EP838125LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a 2-isopropylmalate synthase (leuA), partial CSB-YP838126LPY
CSB-EP838126LPY
CSB-BP838126LPY
CSB-MP838126LPY
CSB-EP838126LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Tyrosine recombinase XerD (xerD) CSB-YP838127LPY
CSB-EP838127LPY
CSB-BP838127LPY
CSB-MP838127LPY
CSB-EP838127LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Histidinol-phosphate aminotransferase (hisC) CSB-YP838128LPY
CSB-EP838128LPY
CSB-BP838128LPY
CSB-MP838128LPY
CSB-EP838128LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Pantothenate synthetase (panC) CSB-YP838129LPY
CSB-EP838129LPY
CSB-BP838129LPY
CSB-MP838129LPY
CSB-EP838129LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Transcriptional regulator MntR (mntR) CSB-YP838130LPY
CSB-EP838130LPY
CSB-BP838130LPY
CSB-MP838130LPY
CSB-EP838130LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Peptide methionine sulfoxide reductase MsrB (msrB) CSB-YP838131LPY
CSB-EP838131LPY
CSB-BP838131LPY
CSB-MP838131LPY
CSB-EP838131LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP838132LPY
CSB-EP838132LPY
CSB-BP838132LPY
CSB-MP838132LPY
CSB-EP838132LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Methionyl-tRNA formyltransferase (fmt) CSB-YP838133LPY
CSB-EP838133LPY
CSB-BP838133LPY
CSB-MP838133LPY
CSB-EP838133LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Ribosome maturation factor RimM (rimM) CSB-YP838134LPY
CSB-EP838134LPY
CSB-BP838134LPY
CSB-MP838134LPY
CSB-EP838134LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP838135LPY
CSB-EP838135LPY
CSB-BP838135LPY
CSB-MP838135LPY
CSB-EP838135LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a UPF0435 protein lmo1707 (lmo1707) CSB-YP838136LPY
CSB-EP838136LPY
CSB-BP838136LPY
CSB-MP838136LPY
CSB-EP838136LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Elongation factor Ts (tsf) CSB-YP838137LPY
CSB-EP838137LPY
CSB-BP838137LPY
CSB-MP838137LPY
CSB-EP838137LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a tRNA (guanine-N (7)-)-methyltransferase (trmB) CSB-YP838138LPY
CSB-EP838138LPY
CSB-BP838138LPY
CSB-MP838138LPY
CSB-EP838138LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a 30S ribosomal protein S4 (rpsD) CSB-YP838139LPY
CSB-EP838139LPY
CSB-BP838139LPY
CSB-MP838139LPY
CSB-EP838139LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Probable thiol peroxidase (tpx) CSB-YP838140LPY
CSB-EP838140LPY
CSB-BP838140LPY
CSB-MP838140LPY
CSB-EP838140LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Dephospho-CoA kinase (coaE) CSB-YP838141LPY
CSB-EP838141LPY
CSB-BP838141LPY
CSB-MP838141LPY
CSB-EP838141LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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