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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Listeria monocytogenes serovar 1/2a UPF0758 protein lmo1549 (lmo1549) CSB-YP838142LPY
CSB-EP838142LPY
CSB-BP838142LPY
CSB-MP838142LPY
CSB-EP838142LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a L-lactate dehydrogenase 2 (ldh2) CSB-YP838143LPY
CSB-EP838143LPY
CSB-BP838143LPY
CSB-MP838143LPY
CSB-EP838143LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Putative Holliday junction resolvase (lmo1502) CSB-YP838144LPY
CSB-EP838144LPY
CSB-BP838144LPY
CSB-MP838144LPY
CSB-EP838144LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Probable endonuclease 4 (nfo) CSB-YP838145LPY
CSB-EP838145LPY
CSB-BP838145LPY
CSB-MP838145LPY
CSB-EP838145LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a DNA mismatch repair protein mutL (mutL), partial CSB-YP838146LPY
CSB-EP838146LPY
CSB-BP838146LPY
CSB-MP838146LPY
CSB-EP838146LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP838147LPY
CSB-EP838147LPY
CSB-BP838147LPY
CSB-MP838147LPY
CSB-EP838147LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Proline--tRNA ligase (proS), partial CSB-YP838148LPY
CSB-EP838148LPY
CSB-BP838148LPY
CSB-MP838148LPY
CSB-EP838148LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Cell division suppressor protein YneA (yneA) CSB-YP838149LPY
CSB-EP838149LPY
CSB-BP838149LPY
CSB-MP838149LPY
CSB-EP838149LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a ATP-dependent protease subunit HslV (hslV) CSB-YP838150LPY
CSB-EP838150LPY
CSB-BP838150LPY
CSB-MP838150LPY
CSB-EP838150LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Glutamate racemase (murI) CSB-YP838151LPY
CSB-EP838151LPY
CSB-BP838151LPY
CSB-MP838151LPY
CSB-EP838151LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Cobyric acid synthase (cobQ), partial CSB-YP838152LPY
CSB-EP838152LPY
CSB-BP838152LPY
CSB-MP838152LPY
CSB-EP838152LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Cobalamin synthase (cobS), partial CSB-YP838153LPY1
CSB-EP838153LPY1
CSB-BP838153LPY1
CSB-MP838153LPY1
CSB-EP838153LPY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP838154LPY
CSB-EP838154LPY
CSB-BP838154LPY
CSB-MP838154LPY
CSB-EP838154LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Protease HtpX homolog (htpX), partial CSB-YP838155LPY1
CSB-EP838155LPY1
CSB-BP838155LPY1
CSB-MP838155LPY1
CSB-EP838155LPY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a D-alanine--D-alanine ligase (ddl) CSB-YP838156LPY
CSB-EP838156LPY
CSB-BP838156LPY
CSB-MP838156LPY
CSB-EP838156LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS), partial CSB-YP838157LPY
CSB-EP838157LPY
CSB-BP838157LPY
CSB-MP838157LPY
CSB-EP838157LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a ATP phosphoribosyltransferase (hisG) CSB-YP838158LPY
CSB-EP838158LPY
CSB-BP838158LPY
CSB-MP838158LPY
CSB-EP838158LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Tagatose 1,6-diphosphate aldolase (lacD) CSB-YP838159LPY
CSB-EP838159LPY
CSB-BP838159LPY
CSB-MP838159LPY
CSB-EP838159LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Putative sugar uptake protein lmo0424 (lmo0424), partial CSB-YP838160LPY
CSB-EP838160LPY
CSB-BP838160LPY
CSB-MP838160LPY
CSB-EP838160LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Internalin-I (inlI), partial CSB-YP838161LPY
CSB-EP838161LPY
CSB-BP838161LPY
CSB-MP838161LPY
CSB-EP838161LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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