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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Ocimum basilicum Eugenol O-methyltransferase (EOMT1) CSB-YP846353OAAC
CSB-EP846353OAAC
CSB-BP846353OAAC
CSB-MP846353OAAC
CSB-EP846353OAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable WRKY transcription factor 20 (WRKY20), partial CSB-YP846354DOA
CSB-EP846354DOA
CSB-BP846354DOA
CSB-MP846354DOA
CSB-EP846354DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brassica napus 3-oxoacyl-[acyl-carrier-protein] reductase 2, chloroplastic (bkr2) CSB-YP846355BWD
CSB-EP846355BWD
CSB-BP846355BWD
CSB-MP846355BWD
CSB-EP846355BWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica N-carbamoylputrescine amidase (CPA) CSB-YP846356OFG
CSB-EP846356OFG
CSB-BP846356OFG
CSB-MP846356OFG
CSB-EP846356OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Zinc finger CCCH domain-containing protein 40 (At3g21810) CSB-YP846357DOA
CSB-EP846357DOA
CSB-BP846357DOA
CSB-MP846357DOA
CSB-EP846357DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Coatomer subunit delta (At5g05010), partial CSB-YP846358DOA
CSB-EP846358DOA
CSB-BP846358DOA
CSB-MP846358DOA
CSB-EP846358DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii Soluble inorganic pyrophosphatase 1, chloroplastic (ppa1), partial CSB-YP846359DSJ
CSB-EP846359DSJ
CSB-BP846359DSJ
CSB-MP846359DSJ
CSB-EP846359DSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ubiquitin-conjugating enzyme E2 variant 1A (UEV1A) CSB-YP846360DOA
CSB-EP846360DOA
CSB-BP846360DOA
CSB-MP846360DOA
CSB-EP846360DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable protein phosphatase 2C 51 (At3g63340), partial CSB-YP846361DOA
CSB-EP846361DOA
CSB-BP846361DOA
CSB-MP846361DOA
CSB-EP846361DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable methyltransferase PMT3 (At4g14360), partial CSB-YP846362DOA
CSB-EP846362DOA
CSB-BP846362DOA
CSB-MP846362DOA
CSB-EP846362DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Glucan endo-1,3-beta-glucosidase 6 (At5g58090) CSB-YP846363DOA
CSB-EP846363DOA
CSB-BP846363DOA
CSB-MP846363DOA
CSB-EP846363DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ribose-phosphate pyrophosphokinase 3, chloroplastic (PRS3) CSB-YP846364DOA
CSB-EP846364DOA
CSB-BP846364DOA
CSB-MP846364DOA
CSB-EP846364DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ent-kaurene oxidase, chloroplastic (KO1), partial CSB-YP846365DOA
CSB-EP846365DOA
CSB-BP846365DOA
CSB-MP846365DOA
CSB-EP846365DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Stromal cell-derived factor 2-like protein (At2g25110) CSB-YP846366DOA
CSB-EP846366DOA
CSB-BP846366DOA
CSB-MP846366DOA
CSB-EP846366DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Dof zinc finger protein DOF5.2 (DOF5.2) CSB-YP846367DOA
CSB-EP846367DOA
CSB-BP846367DOA
CSB-MP846367DOA
CSB-EP846367DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein NSP-INTERACTING KINASE 3 (NIK3), partial CSB-YP846368DOA
CSB-EP846368DOA
CSB-BP846368DOA
CSB-MP846368DOA
CSB-EP846368DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative RNA polymerase II transcriptional coactivator (T13F2.2) CSB-YP846369CXY
CSB-EP846369CXY
CSB-BP846369CXY
CSB-MP846369CXY
CSB-EP846369CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Gibberellin receptor GID1C (GID1C) CSB-YP846370DOA
CSB-EP846370DOA
CSB-BP846370DOA
CSB-MP846370DOA
CSB-EP846370DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At1g07590, mitochondrial (At1g07590), partial CSB-YP846371DOA
CSB-EP846371DOA
CSB-BP846371DOA
CSB-MP846371DOA
CSB-EP846371DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cytochrome P450 85A2 (CYP85A2), partial CSB-YP846372DOA
CSB-EP846372DOA
CSB-BP846372DOA
CSB-MP846372DOA
CSB-EP846372DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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