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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Listeria monocytogenes serovar 1/2a Protein CrcB homolog 1 (crcB1), partial CSB-YP855750LPY1
CSB-EP855750LPY1
CSB-BP855750LPY1
CSB-MP855750LPY1
CSB-EP855750LPY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) CSB-YP855751LPY
CSB-EP855751LPY
CSB-BP855751LPY
CSB-MP855751LPY
CSB-EP855751LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Deoxyribose-phosphate aldolase (deoC) CSB-YP855752LPY
CSB-EP855752LPY
CSB-BP855752LPY
CSB-MP855752LPY
CSB-EP855752LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Uncharacterized protein Lmo1967 (lmo1967) CSB-YP855753LPY
CSB-EP855753LPY
CSB-BP855753LPY
CSB-MP855753LPY
CSB-EP855753LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Chorismate synthase (aroC) CSB-YP855754LPY
CSB-EP855754LPY
CSB-BP855754LPY
CSB-MP855754LPY
CSB-EP855754LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a CCA-adding enzyme (cca) CSB-YP855755LPY
CSB-EP855755LPY
CSB-BP855755LPY
CSB-MP855755LPY
CSB-EP855755LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Xanthine phosphoribosyltransferase (xpt) CSB-YP855756LPY
CSB-EP855756LPY
CSB-BP855756LPY
CSB-MP855756LPY
CSB-EP855756LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a UPF0346 protein lmo1857 (lmo1857) CSB-YP855757LPY
CSB-EP855757LPY
CSB-BP855757LPY
CSB-MP855757LPY
CSB-EP855757LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Probable multidrug resistance protein norM (norM), partial CSB-YP855758LPY
CSB-EP855758LPY
CSB-BP855758LPY
CSB-MP855758LPY
CSB-EP855758LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Dihydroorotate dehydrogenase B (NAD (+)), electron transfer subunit (pyrK) CSB-YP855759LPY
CSB-EP855759LPY
CSB-BP855759LPY
CSB-MP855759LPY
CSB-EP855759LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Putative ribosome biogenesis GTPase RsgA 2 (rsgA2) CSB-YP855760LPY
CSB-EP855760LPY
CSB-BP855760LPY
CSB-MP855760LPY
CSB-EP855760LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP855761LPY
CSB-EP855761LPY
CSB-BP855761LPY
CSB-MP855761LPY
CSB-EP855761LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Phosphoribosylamine--glycine ligase (purD) CSB-YP855762LPY
CSB-EP855762LPY
CSB-BP855762LPY
CSB-MP855762LPY
CSB-EP855762LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Regulatory protein recX (recX) CSB-YP855763LPY
CSB-EP855763LPY
CSB-BP855763LPY
CSB-MP855763LPY
CSB-EP855763LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Leucine--tRNA ligase (leuS), partial CSB-YP855764LPY
CSB-EP855764LPY
CSB-BP855764LPY
CSB-MP855764LPY
CSB-EP855764LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a UPF0354 protein lmo1608 (lmo1608) CSB-YP855765LPY
CSB-EP855765LPY
CSB-BP855765LPY
CSB-MP855765LPY
CSB-EP855765LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Arginine biosynthesis bifunctional protein ArgJ (argJ) CSB-YP855766LPY
CSB-EP855766LPY
CSB-BP855766LPY
CSB-MP855766LPY
CSB-EP855766LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP855767LPY
CSB-EP855767LPY
CSB-BP855767LPY
CSB-MP855767LPY
CSB-EP855767LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Probable GTP-binding protein EngB (engB) CSB-YP855768LPY
CSB-EP855768LPY
CSB-BP855768LPY
CSB-MP855768LPY
CSB-EP855768LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Glycerol kinase (glpK) CSB-YP855769LPY
CSB-EP855769LPY
CSB-BP855769LPY
CSB-MP855769LPY
CSB-EP855769LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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