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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Rickettsia conorii Uncharacterized protein RC0016 (RC0016) CSB-YP856551RMS
CSB-EP856551RMS
CSB-BP856551RMS
CSB-MP856551RMS
CSB-EP856551RMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP856552RKU
CSB-EP856552RKU
CSB-BP856552RKU
CSB-MP856552RKU
CSB-EP856552RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP856553RKU
CSB-EP856553RKU
CSB-BP856553RKU
CSB-MP856553RKU
CSB-EP856553RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Diaminopimelate epimerase (dapF) CSB-YP856554RKU
CSB-EP856554RKU
CSB-BP856554RKU
CSB-MP856554RKU
CSB-EP856554RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti DNA translocase FtsK (ftsK), partial CSB-YP856555RKU
CSB-EP856555RKU
CSB-BP856555RKU
CSB-MP856555RKU
CSB-EP856555RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Gamma-glutamyl phosphate reductase (proA) CSB-YP856556RKU
CSB-EP856556RKU
CSB-BP856556RKU
CSB-MP856556RKU
CSB-EP856556RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP856557RKU
CSB-EP856557RKU
CSB-BP856557RKU
CSB-MP856557RKU
CSB-EP856557RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP856558RKU
CSB-EP856558RKU
CSB-BP856558RKU
CSB-MP856558RKU
CSB-EP856558RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP856559RKU
CSB-EP856559RKU
CSB-BP856559RKU
CSB-MP856559RKU
CSB-EP856559RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Probable transcriptional regulatory protein R02753 (R02753) CSB-YP856560RKU
CSB-EP856560RKU
CSB-BP856560RKU
CSB-MP856560RKU
CSB-EP856560RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Tyrosine recombinase XerD (xerD) CSB-YP856561RKU
CSB-EP856561RKU
CSB-BP856561RKU
CSB-MP856561RKU
CSB-EP856561RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Arginine biosynthesis bifunctional protein ArgJ (argJ) CSB-YP856562RKU
CSB-EP856562RKU
CSB-BP856562RKU
CSB-MP856562RKU
CSB-EP856562RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Putative D-xylulose reductase (R02526) CSB-YP856563RKU
CSB-EP856563RKU
CSB-BP856563RKU
CSB-MP856563RKU
CSB-EP856563RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti 6,7-dimethyl-8-ribityllumazine synthase 2 (ribH2) CSB-YP856564RKU
CSB-EP856564RKU
CSB-BP856564RKU
CSB-MP856564RKU
CSB-EP856564RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti D-alanine--D-alanine ligase (ddl) CSB-YP856565RKU
CSB-EP856565RKU
CSB-BP856565RKU
CSB-MP856565RKU
CSB-EP856565RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Cobyric acid synthase (cobQ) CSB-YP856566RKU
CSB-EP856566RKU
CSB-BP856566RKU
CSB-MP856566RKU
CSB-EP856566RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP856567RKU
CSB-EP856567RKU
CSB-BP856567RKU
CSB-MP856567RKU
CSB-EP856567RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP856568RKU
CSB-EP856568RKU
CSB-BP856568RKU
CSB-MP856568RKU
CSB-EP856568RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Indole-3-glycerol phosphate synthase (trpC) CSB-YP856569RKU
CSB-EP856569RKU
CSB-BP856569RKU
CSB-MP856569RKU
CSB-EP856569RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Methionine--tRNA ligase (metG), partial CSB-YP856570RKU
CSB-EP856570RKU
CSB-BP856570RKU
CSB-MP856570RKU
CSB-EP856570RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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