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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhizobium meliloti Orotate phosphoribosyltransferase (pyrE) CSB-YP856591RKU
CSB-EP856591RKU
CSB-BP856591RKU
CSB-MP856591RKU
CSB-EP856591RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP856592RKU
CSB-EP856592RKU
CSB-BP856592RKU
CSB-MP856592RKU
CSB-EP856592RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Lipoprotein signal peptidase (lspA), partial CSB-YP856593RKU1
CSB-EP856593RKU1
CSB-BP856593RKU1
CSB-MP856593RKU1
CSB-EP856593RKU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti 50S ribosomal protein L20 (rplT) CSB-YP856594RKU
CSB-EP856594RKU
CSB-BP856594RKU
CSB-MP856594RKU
CSB-EP856594RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP856595RKU
CSB-EP856595RKU
CSB-BP856595RKU
CSB-MP856595RKU
CSB-EP856595RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP856596RKU
CSB-EP856596RKU
CSB-BP856596RKU
CSB-MP856596RKU
CSB-EP856596RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Adenine deaminase (R00132) CSB-YP856597RKU
CSB-EP856597RKU
CSB-BP856597RKU
CSB-MP856597RKU
CSB-EP856597RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP856598RKU
CSB-EP856598RKU
CSB-BP856598RKU
CSB-MP856598RKU
CSB-EP856598RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Tryptophan synthase alpha chain (trpA) CSB-YP856599RKU
CSB-EP856599RKU
CSB-BP856599RKU
CSB-MP856599RKU
CSB-EP856599RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Methylthioribose-1-phosphate isomerase (mtnA) CSB-YP856600RKU
CSB-EP856600RKU
CSB-BP856600RKU
CSB-MP856600RKU
CSB-EP856600RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Probable septum site-determining protein MinC (minC) CSB-YP856601RKU
CSB-EP856601RKU
CSB-BP856601RKU
CSB-MP856601RKU
CSB-EP856601RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Fe (3+) ions import ATP-binding protein FbpC 2 (fbpC2) CSB-YP856602RKU
CSB-EP856602RKU
CSB-BP856602RKU
CSB-MP856602RKU
CSB-EP856602RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti UPF0194 membrane protein RB0873 (RB0873) CSB-YP856603RKU
CSB-EP856603RKU
CSB-BP856603RKU
CSB-MP856603RKU
CSB-EP856603RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Peptidase T-like protein RB0614 (RB0614) CSB-YP856604RKU
CSB-EP856604RKU
CSB-BP856604RKU
CSB-MP856604RKU
CSB-EP856604RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Fe (3+) ions import ATP-binding protein FbpC 1 (fbpC1) CSB-YP856605RKU
CSB-EP856605RKU
CSB-BP856605RKU
CSB-MP856605RKU
CSB-EP856605RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Potassium-transporting ATPase C chain (kdpC), partial CSB-YP856606RKU
CSB-EP856606RKU
CSB-BP856606RKU
CSB-MP856606RKU
CSB-EP856606RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Peptide methionine sulfoxide reductase MsrA 3 (msrA3) CSB-YP856607RKU
CSB-EP856607RKU
CSB-BP856607RKU
CSB-MP856607RKU
CSB-EP856607RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Chemotaxis response regulator protein-glutamate methylesterase of group 3 operon (cheB3) CSB-YP856608RKU
CSB-EP856608RKU
CSB-BP856608RKU
CSB-MP856608RKU
CSB-EP856608RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (nodM), partial CSB-YP856609RKU
CSB-EP856609RKU
CSB-BP856609RKU
CSB-MP856609RKU
CSB-EP856609RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Probable ubiquitin carboxyl-terminal hydrolase FAF-X (USP9X), partial CSB-YP856610HU
CSB-EP856610HU
CSB-BP856610HU
CSB-MP856610HU
CSB-EP856610HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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