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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Caulobacter crescentus NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP860943DQO1
CSB-EP860943DQO1
CSB-BP860943DQO1
CSB-MP860943DQO1
CSB-EP860943DQO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Ribosome-recycling factor (frr) CSB-YP860944DQO
CSB-EP860944DQO
CSB-BP860944DQO
CSB-MP860944DQO
CSB-EP860944DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus LexA repressor (lexA) CSB-YP860945DQO
CSB-EP860945DQO
CSB-BP860945DQO
CSB-MP860945DQO
CSB-EP860945DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Peptide chain release factor 2 (prfB) CSB-YP860946DQO
CSB-EP860946DQO
CSB-BP860946DQO
CSB-MP860946DQO
CSB-EP860946DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus 30S ribosomal protein S18 (rpsR) CSB-YP860947DQO
CSB-EP860947DQO
CSB-BP860947DQO
CSB-MP860947DQO
CSB-EP860947DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus N-succinylglutamate 5-semialdehyde dehydrogenase 2 (astD2) CSB-YP860948DQO
CSB-EP860948DQO
CSB-BP860948DQO
CSB-MP860948DQO
CSB-EP860948DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP860949DQO
CSB-EP860949DQO
CSB-BP860949DQO
CSB-MP860949DQO
CSB-EP860949DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Ribosomal protein L11 methyltransferase (prmA) CSB-YP860950DQO
CSB-EP860950DQO
CSB-BP860950DQO
CSB-MP860950DQO
CSB-EP860950DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP860951DQO
CSB-EP860951DQO
CSB-BP860951DQO
CSB-MP860951DQO
CSB-EP860951DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus K (+)-insensitive pyrophosphate-energized proton pump (hppA), partial CSB-YP860952DQO
CSB-EP860952DQO
CSB-BP860952DQO
CSB-MP860952DQO
CSB-EP860952DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Uncharacterized RNA methyltransferase CC_1326 (CC_1326) CSB-YP860953DQO
CSB-EP860953DQO
CSB-BP860953DQO
CSB-MP860953DQO
CSB-EP860953DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus 50S ribosomal protein L30 (rpmD) CSB-YP860954DQO
CSB-EP860954DQO
CSB-BP860954DQO
CSB-MP860954DQO
CSB-EP860954DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus 50S ribosomal protein L5 (rplE) CSB-YP860955DQO
CSB-EP860955DQO
CSB-BP860955DQO
CSB-MP860955DQO
CSB-EP860955DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus 50S ribosomal protein L22 (rplV) CSB-YP860956DQO
CSB-EP860956DQO
CSB-BP860956DQO
CSB-MP860956DQO
CSB-EP860956DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus UPF0178 protein CC_1215 (CC_1215) CSB-YP860957DQO
CSB-EP860957DQO
CSB-BP860957DQO
CSB-MP860957DQO
CSB-EP860957DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus UPF0176 protein CC_1060 (CC_1060) CSB-YP860958DQO
CSB-EP860958DQO
CSB-BP860958DQO
CSB-MP860958DQO
CSB-EP860958DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Elongation factor 4 (lepA), partial CSB-YP860959DQO
CSB-EP860959DQO
CSB-BP860959DQO
CSB-MP860959DQO
CSB-EP860959DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Release factor glutamine methyltransferase (prmC) CSB-YP860960DQO
CSB-EP860960DQO
CSB-BP860960DQO
CSB-MP860960DQO
CSB-EP860960DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Ribonuclease P protein component (rnpA) CSB-YP860961DQO
CSB-EP860961DQO
CSB-BP860961DQO
CSB-MP860961DQO
CSB-EP860961DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus 50S ribosomal protein L11 (rplK) CSB-YP860962DQO
CSB-EP860962DQO
CSB-BP860962DQO
CSB-MP860962DQO
CSB-EP860962DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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