重組蛋白
重組蛋白質(zhì)是分析生物相互作用的有用工具。在研究實(shí)驗(yàn)室中使用了大量的重組蛋白質(zhì)，包括商業(yè)蛋白質(zhì)和在特定研究項(xiàng)目中生成的蛋白質(zhì)。它們?cè)谠S多應(yīng)用中發(fā)揮著關(guān)鍵作用。
如果您正在尋找具有優(yōu)良質(zhì)量（驗(yàn)證的生物活性，較低的內(nèi)毒素水平和高純度）的重組蛋白質(zhì)來支持您的研究，您來對(duì)地方了。
經(jīng)過18年的發(fā)展，華美生物已經(jīng)建立了從原核到真核的五大系統(tǒng)，用于表達(dá)重組蛋白質(zhì)。我們擁有一系列蛋白質(zhì)表達(dá)和純化技術(shù)，包括基于桿狀病毒外膜蛋白（OMP）的蛋白質(zhì)表達(dá)技術(shù)，以及膜蛋白表達(dá)技術(shù)等。
產(chǎn)品特點(diǎn)
- 超過13,000種重組蛋白覆蓋大多數(shù)基因/靶點(diǎn)；
- 5種表達(dá)系統(tǒng)，包括大腸桿菌（E.coli）、酵母、桿狀病毒感染的昆蟲細(xì)胞、哺乳動(dòng)物細(xì)胞和體外大腸桿菌。而體外大腸桿菌表達(dá)系統(tǒng)是華美生物的獨(dú)特表達(dá)系統(tǒng)。與傳統(tǒng)蛋白表達(dá)系統(tǒng)相比，體外大腸桿菌省略了許多過程，如質(zhì)粒轉(zhuǎn)化、細(xì)胞培養(yǎng)、收集、破碎和離心等，大大提高了工作效率。
- 多種標(biāo)簽，包括His、GST、Flag、MBP等。
- 廣泛應(yīng)用，包括抗原、細(xì)胞分析、結(jié)合分析/蛋白質(zhì)相互作用、細(xì)胞培養(yǎng)-無血清培養(yǎng)基、藥物相關(guān)研究、體外酶活性、ELISA標(biāo)準(zhǔn)及其原料、體內(nèi)研究、質(zhì)譜標(biāo)準(zhǔn)、蛋白芯片、SDS-PAGE控制、蛋白質(zhì)結(jié)構(gòu)分析（晶體/電子顯微鏡）。
- 多種物種，包括人類、小鼠、大鼠、兔子、豚鼠、雞、猴子、豬等。
- 無動(dòng)物副產(chǎn)品的動(dòng)物源性重組蛋白質(zhì)。華美生物已開發(fā)出無動(dòng)物副產(chǎn)品接觸的重組蛋白質(zhì)產(chǎn)品線。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質(zhì)，或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的抗體，請(qǐng)查看華美生物蛋白質(zhì)定制服務(wù)。
通過選擇目標(biāo)名稱的首字母瀏覽產(chǎn)品。
| 產(chǎn)品名稱 | 貨號(hào) | 規(guī)格 |
|---|---|---|
| Recombinant Neisseria meningitidis serogroup A / serotype 4A UPF0324 membrane protein NMA0465 (NMA0465), partial | CSB-YP864426NGF CSB-EP864426NGF CSB-BP864426NGF CSB-MP864426NGF CSB-EP864426NGF-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Neisseria meningitidis serogroup A / serotype 4A tRNA pseudouridine synthase A (truA) | CSB-YP864427NGF CSB-EP864427NGF CSB-BP864427NGF CSB-MP864427NGF CSB-EP864427NGF-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Neisseria meningitidis serogroup A / serotype 4A Thiazole synthase (thiG) | CSB-YP864428NGF CSB-EP864428NGF CSB-BP864428NGF CSB-MP864428NGF CSB-EP864428NGF-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Neisseria meningitidis serogroup A / serotype 4A Peptide methionine sulfoxide reductase MsrA/MsrB (msrAB), partial | CSB-YP864429NGF CSB-EP864429NGF CSB-BP864429NGF CSB-MP864429NGF CSB-EP864429NGF-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Neisseria meningitidis serogroup A / serotype 4A Phosphoglycerate kinase (pgk) | CSB-YP864430NGF CSB-EP864430NGF CSB-BP864430NGF CSB-MP864430NGF CSB-EP864430NGF-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Neisseria meningitidis serogroup A / serotype 4A 50S ribosomal protein L23 (rplW) | CSB-YP864431NGF CSB-EP864431NGF CSB-BP864431NGF CSB-MP864431NGF CSB-EP864431NGF-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Neisseria meningitidis serogroup A / serotype 4A Valine--tRNA ligase (valS), partial | CSB-YP864432NGF CSB-EP864432NGF CSB-BP864432NGF CSB-MP864432NGF CSB-EP864432NGF-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Neisseria meningitidis serogroup A / serotype 4A Undecaprenyl pyrophosphate synthase (uppS) | CSB-YP864433NGF CSB-EP864433NGF CSB-BP864433NGF CSB-MP864433NGF CSB-EP864433NGF-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Neisseria meningitidis serogroup A / serotype 4A NADH-quinone oxidoreductase subunit L (nuoL), partial | CSB-YP864434NGF CSB-EP864434NGF CSB-BP864434NGF CSB-MP864434NGF CSB-EP864434NGF-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Neisseria meningitidis serogroup B Argininosuccinate synthase (argG) | CSB-YP864435NGG CSB-EP864435NGG CSB-BP864435NGG CSB-MP864435NGG CSB-EP864435NGG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Neisseria meningitidis serogroup B Protein SlyX homolog (slyX) | CSB-YP864436NGG CSB-EP864436NGG CSB-BP864436NGG CSB-MP864436NGG CSB-EP864436NGG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Neisseria meningitidis serogroup B 33 kDa chaperonin (hslO) | CSB-YP864437NGG CSB-EP864437NGG CSB-BP864437NGG CSB-MP864437NGG CSB-EP864437NGG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Neisseria meningitidis serogroup B Probable chromosome-partitioning protein parB (parB) | CSB-YP864438NGG CSB-EP864438NGG CSB-BP864438NGG CSB-MP864438NGG CSB-EP864438NGG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Neisseria meningitidis serogroup B ATP synthase subunit beta (atpD) | CSB-YP864439NGG CSB-EP864439NGG CSB-BP864439NGG CSB-MP864439NGG CSB-EP864439NGG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Neisseria meningitidis serogroup B Chromosomal replication initiator protein DnaA (dnaA), partial | CSB-YP864440NGG CSB-EP864440NGG CSB-BP864440NGG CSB-MP864440NGG CSB-EP864440NGG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Neisseria meningitidis serogroup B (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB) | CSB-YP864441NGG CSB-EP864441NGG CSB-BP864441NGG CSB-MP864441NGG CSB-EP864441NGG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Neisseria meningitidis serogroup B DNA polymerase III subunit alpha (dnaE), partial | CSB-YP864442NGG CSB-EP864442NGG CSB-BP864442NGG CSB-MP864442NGG CSB-EP864442NGG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Neisseria meningitidis serogroup B S-adenosylmethionine synthase (metK) | CSB-YP864443NGG CSB-EP864443NGG CSB-BP864443NGG CSB-MP864443NGG CSB-EP864443NGG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Neisseria meningitidis serogroup B Peptide chain release factor 1 (prfA) | CSB-YP864444NGG CSB-EP864444NGG CSB-BP864444NGG CSB-MP864444NGG CSB-EP864444NGG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Neisseria meningitidis serogroup B UPF0237 protein NMB1653 (NMB1653) | CSB-YP864445NGG CSB-EP864445NGG CSB-BP864445NGG CSB-MP864445NGG CSB-EP864445NGG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。
第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條：如何選擇合適的表達(dá)載體?
表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。
根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022
