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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus halodurans Cell division protein ZapA (zapA) CSB-YP864506BQS
CSB-EP864506BQS
CSB-BP864506BQS
CSB-MP864506BQS
CSB-EP864506BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Ketol-acid reductoisomerase (ilvC) CSB-YP864507BQS
CSB-EP864507BQS
CSB-BP864507BQS
CSB-MP864507BQS
CSB-EP864507BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Probable GTP-binding protein EngB (engB) CSB-YP864508BQS
CSB-EP864508BQS
CSB-BP864508BQS
CSB-MP864508BQS
CSB-EP864508BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Delta-aminolevulinic acid dehydratase (hemB) CSB-YP864509BQS
CSB-EP864509BQS
CSB-BP864509BQS
CSB-MP864509BQS
CSB-EP864509BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Molybdopterin synthase catalytic subunit (moaE) CSB-YP864510BQS
CSB-EP864510BQS
CSB-BP864510BQS
CSB-MP864510BQS
CSB-EP864510BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Protein CrcB homolog 1 (crcB1), partial CSB-YP864511BQS1
CSB-EP864511BQS1
CSB-BP864511BQS1
CSB-MP864511BQS1
CSB-EP864511BQS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Carbamoyl-phosphate synthase arginine-specific large chain (carB), partial CSB-YP864512BQS
CSB-EP864512BQS
CSB-BP864512BQS
CSB-MP864512BQS
CSB-EP864512BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Putative membrane protein insertion efficiency factor (BH2828) CSB-YP864513BQS
CSB-EP864513BQS
CSB-BP864513BQS
CSB-MP864513BQS
CSB-EP864513BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans N utilization substance protein B homolog (nusB) CSB-YP864514BQS
CSB-EP864514BQS
CSB-BP864514BQS
CSB-MP864514BQS
CSB-EP864514BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans DNA repair protein recN (recN), partial CSB-YP864515BQS
CSB-EP864515BQS
CSB-BP864515BQS
CSB-MP864515BQS
CSB-EP864515BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Protease HtpX homolog (htpX), partial CSB-YP864516BQS1
CSB-EP864516BQS1
CSB-BP864516BQS1
CSB-MP864516BQS1
CSB-EP864516BQS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Peptide deformylase (def) CSB-YP864517BQS
CSB-EP864517BQS
CSB-BP864517BQS
CSB-MP864517BQS
CSB-EP864517BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans UPF0637 protein BH2637 (BH2637) CSB-YP864518BQS
CSB-EP864518BQS
CSB-BP864518BQS
CSB-MP864518BQS
CSB-EP864518BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Protoheme IX farnesyltransferase (ctaB), partial CSB-YP864519BQS
CSB-EP864519BQS
CSB-BP864519BQS
CSB-MP864519BQS
CSB-EP864519BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP864520BQS
CSB-EP864520BQS
CSB-BP864520BQS
CSB-MP864520BQS
CSB-EP864520BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Lipoprotein signal peptidase (lspA), partial CSB-YP864521BQS1
CSB-EP864521BQS1
CSB-BP864521BQS1
CSB-MP864521BQS1
CSB-EP864521BQS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Dihydroorotate dehydrogenase B (NAD (+)), catalytic subunit (pyrD) CSB-YP864522BQS
CSB-EP864522BQS
CSB-BP864522BQS
CSB-MP864522BQS
CSB-EP864522BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP864523BQS
CSB-EP864523BQS
CSB-BP864523BQS
CSB-MP864523BQS
CSB-EP864523BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Ribonuclease 3 (rnc) CSB-YP864524BQS
CSB-EP864524BQS
CSB-BP864524BQS
CSB-MP864524BQS
CSB-EP864524BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans ATP-dependent protease subunit HslV (hslV) CSB-YP864525BQS
CSB-EP864525BQS
CSB-BP864525BQS
CSB-MP864525BQS
CSB-EP864525BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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