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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Protein ROOT HAIR DEFECTIVE 3 homolog 1 (At1g72960), partial CSB-YP865967DOA1
CSB-EP865967DOA1
CSB-BP865967DOA1
CSB-MP865967DOA1
CSB-EP865967DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At1g52640, mitochondrial (At1g52640), partial CSB-YP865968DOA
CSB-EP865968DOA
CSB-BP865968DOA
CSB-MP865968DOA
CSB-EP865968DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucumis sativus Ethylene receptor 1 (ETR1), partial CSB-YP865969CXP1
CSB-EP865969CXP1
CSB-BP865969CXP1
CSB-MP865969CXP1
CSB-EP865969CXP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Profilin-2 (PRO2) CSB-YP865970NHE
CSB-EP865970NHE
CSB-BP865970NHE
CSB-MP865970NHE
CSB-EP865970NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative disease resistance RPP13-like protein 3 (RPP13L3), partial CSB-YP865971DOA
CSB-EP865971DOA
CSB-BP865971DOA
CSB-MP865971DOA
CSB-EP865971DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Deoxyuridine 5'-triphosphate nucleotidohydrolase (DUT) CSB-YP865972DOA
CSB-EP865972DOA
CSB-BP865972DOA
CSB-MP865972DOA
CSB-EP865972DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Zinc finger A20 and AN1 domain-containing stress-associated protein 10 (SAP10) CSB-YP865973DOA
CSB-EP865973DOA
CSB-BP865973DOA
CSB-MP865973DOA
CSB-EP865973DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cytochrome P450 71A21 (CYP71A21), partial CSB-YP865974DOA
CSB-EP865974DOA
CSB-BP865974DOA
CSB-MP865974DOA
CSB-EP865974DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative transcription elongation factor SPT5 homolog 1 (At4g08350), partial CSB-YP865975DOA
CSB-EP865975DOA
CSB-BP865975DOA
CSB-MP865975DOA
CSB-EP865975DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Arabinogalactan peptide 13 (AGP13) CSB-YP865976DOA
CSB-EP865976DOA
CSB-BP865976DOA
CSB-MP865976DOA
CSB-EP865976DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative pentatricopeptide repeat-containing protein At3g47840 (PCMP-E43), partial CSB-YP865977DOA
CSB-EP865977DOA
CSB-BP865977DOA
CSB-MP865977DOA
CSB-EP865977DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ABC transporter A family member 4 (ABCA4), partial CSB-YP865978DOA
CSB-EP865978DOA
CSB-BP865978DOA
CSB-MP865978DOA
CSB-EP865978DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Heat shock 70 kDa protein 6, chloroplastic (HSP70-6), partial CSB-YP865979DOA
CSB-EP865979DOA
CSB-BP865979DOA
CSB-MP865979DOA
CSB-EP865979DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Phosphoinositide phospholipase C 8 (PLC8), partial CSB-YP865980DOA
CSB-EP865980DOA
CSB-BP865980DOA
CSB-MP865980DOA
CSB-EP865980DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription initiation factor IIF subunit alpha (RAP74), partial CSB-YP865981DOA
CSB-EP865981DOA
CSB-BP865981DOA
CSB-MP865981DOA
CSB-EP865981DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable cyclic nucleotide-gated ion channel 16 (CNGC16), partial CSB-YP865982DOA1
CSB-EP865982DOA1
CSB-BP865982DOA1
CSB-MP865982DOA1
CSB-EP865982DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Calcineurin B-like protein 7 (CBL7) CSB-YP865983DOA
CSB-EP865983DOA
CSB-BP865983DOA
CSB-MP865983DOA
CSB-EP865983DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative protein phosphatase 2C 53 (At4g08260) CSB-YP865984DOA
CSB-EP865984DOA
CSB-BP865984DOA
CSB-MP865984DOA
CSB-EP865984DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At4g30700 (DYW9), partial CSB-YP865985DOA
CSB-EP865985DOA
CSB-BP865985DOA
CSB-MP865985DOA
CSB-EP865985DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Photosystem I reaction center subunit VI-2, chloroplastic (PSAH2), partial CSB-YP865986DOA1
CSB-EP865986DOA1
CSB-BP865986DOA1
CSB-MP865986DOA1
CSB-EP865986DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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