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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Protein FAR-RED IMPAIRED RESPONSE 1 (FAR1), partial CSB-YP866007DOA
CSB-EP866007DOA
CSB-BP866007DOA
CSB-MP866007DOA
CSB-EP866007DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana IAA-amino acid hydrolase ILR1-like 5 (ILL5) CSB-YP866008DOA
CSB-EP866008DOA
CSB-BP866008DOA
CSB-MP866008DOA
CSB-EP866008DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana GDT1-like protein 5 (At1g68650), partial CSB-YP866009DOA1
CSB-EP866009DOA1
CSB-BP866009DOA1
CSB-MP866009DOA1
CSB-EP866009DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Sugar transport protein 9 (STP9), partial CSB-YP866010DOA
CSB-EP866010DOA
CSB-BP866010DOA
CSB-MP866010DOA
CSB-EP866010DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative purine permease 20 (PUP20), partial CSB-YP866011DOA
CSB-EP866011DOA
CSB-BP866011DOA
CSB-MP866011DOA
CSB-EP866011DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Secretory carrier-associated membrane protein 5 (SCAMP5), partial CSB-YP866012DOA1
CSB-EP866012DOA1
CSB-BP866012DOA1
CSB-MP866012DOA1
CSB-EP866012DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable alpha-mannosidase I MNS5 (MNS5), partial CSB-YP866013DOA
CSB-EP866013DOA
CSB-BP866013DOA
CSB-MP866013DOA
CSB-EP866013DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Aquaporin PIP 1-3 (PIP1-3), partial CSB-YP866014OFG1
CSB-EP866014OFG1
CSB-BP866014OFG1
CSB-MP866014OFG1
CSB-EP866014OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Octanoyltransferase (LIP2) CSB-YP866015DOA
CSB-EP866015DOA
CSB-BP866015DOA
CSB-MP866015DOA
CSB-EP866015DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycyrrhiza glabra Cycloartenol synthase (GgCAS1), partial CSB-YP866016GCAJ
CSB-EP866016GCAJ
CSB-BP866016GCAJ
CSB-MP866016GCAJ
CSB-EP866016GCAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein FANTASTIC FOUR 1 (FAF1) CSB-YP866017DOA
CSB-EP866017DOA
CSB-BP866017DOA
CSB-MP866017DOA
CSB-EP866017DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable purine permease 4 (PUP4), partial CSB-YP866018DOA
CSB-EP866018DOA
CSB-BP866018DOA
CSB-MP866018DOA
CSB-EP866018DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At1g10270 (GRP23), partial CSB-YP866019DOA
CSB-EP866019DOA
CSB-BP866019DOA
CSB-MP866019DOA
CSB-EP866019DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box/kelch-repeat protein At1g61540 (At1g61540) CSB-YP866020DOA
CSB-EP866020DOA
CSB-BP866020DOA
CSB-MP866020DOA
CSB-EP866020DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Vesicle-associated protein 1-4 (PVA14), partial CSB-YP866021DOA
CSB-EP866021DOA
CSB-BP866021DOA
CSB-MP866021DOA
CSB-EP866021DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Nuclear transcription factor Y subunit A-5 (NFYA5) CSB-YP866022DOA
CSB-EP866022DOA
CSB-BP866022DOA
CSB-MP866022DOA
CSB-EP866022DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At1g05600 (At1g05600), partial CSB-YP866023DOA
CSB-EP866023DOA
CSB-BP866023DOA
CSB-MP866023DOA
CSB-EP866023DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Mechanosensitive ion channel protein 6 (MSL6), partial CSB-YP866024DOA
CSB-EP866024DOA
CSB-BP866024DOA
CSB-MP866024DOA
CSB-EP866024DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Receptor protein kinase CLAVATA1 (CLV1), partial CSB-YP866025DOA
CSB-EP866025DOA
CSB-BP866025DOA
CSB-MP866025DOA
CSB-EP866025DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana HVA22-like protein b (HVA22B), partial CSB-YP866026DOA1
CSB-EP866026DOA1
CSB-BP866026DOA1
CSB-MP866026DOA1
CSB-EP866026DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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