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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana Probable pectinesterase/pectinesterase inhibitor 58 (PME58), partial CSB-YP866944DOA
CSB-EP866944DOA
CSB-BP866944DOA
CSB-MP866944DOA
CSB-EP866944DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative fimbrin-like protein 3 (FIM3), partial CSB-YP866945DOA
CSB-EP866945DOA
CSB-BP866945DOA
CSB-MP866945DOA
CSB-EP866945DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor ERF025 (ERF025) CSB-YP866946DOA
CSB-EP866946DOA
CSB-BP866946DOA
CSB-MP866946DOA
CSB-EP866946DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Oligopeptide transporter 9 (OPT9), partial CSB-YP866947DOA
CSB-EP866947DOA
CSB-BP866947DOA
CSB-MP866947DOA
CSB-EP866947DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ABC transporter F family member 1 (ABCF1), partial CSB-YP866948DOA
CSB-EP866948DOA
CSB-BP866948DOA
CSB-MP866948DOA
CSB-EP866948DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cyclin-C1-1 (CYCC1-1) CSB-YP866949DOA
CSB-EP866949DOA
CSB-BP866949DOA
CSB-MP866949DOA
CSB-EP866949DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana U-box domain-containing protein 38 (PUB38), partial CSB-YP866950DOA
CSB-EP866950DOA
CSB-BP866950DOA
CSB-MP866950DOA
CSB-EP866950DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative FBD-associated F-box protein At5g56820 (At5g56820) CSB-YP866951DOA
CSB-EP866951DOA
CSB-BP866951DOA
CSB-MP866951DOA
CSB-EP866951DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/FBD/LRR-repeat protein At5g56570 (At5g56570) CSB-YP866952DOA
CSB-EP866952DOA
CSB-BP866952DOA
CSB-MP866952DOA
CSB-EP866952DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 60S ribosomal protein L26-2 (RPL26B) CSB-YP866953DOA
CSB-EP866953DOA
CSB-BP866953DOA
CSB-MP866953DOA
CSB-EP866953DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable pectinesterase/pectinesterase inhibitor 61 (PME61), partial CSB-YP866954DOA1
CSB-EP866954DOA1
CSB-BP866954DOA1
CSB-MP866954DOA1
CSB-EP866954DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable ubiquitin-conjugating enzyme E2 33 (UBC33), partial CSB-YP866955DOA1
CSB-EP866955DOA1
CSB-BP866955DOA1
CSB-MP866955DOA1
CSB-EP866955DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana B3 domain-containing protein At5g18090 (At5g18090) CSB-YP866956DOA
CSB-EP866956DOA
CSB-BP866956DOA
CSB-MP866956DOA
CSB-EP866956DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized protein At5g39570 (At5g39570) CSB-YP866957DOA
CSB-EP866957DOA
CSB-BP866957DOA
CSB-MP866957DOA
CSB-EP866957DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative small ubiquitin-related modifier 6 (SUMO6) CSB-YP866958DOA
CSB-EP866958DOA
CSB-BP866958DOA
CSB-MP866958DOA
CSB-EP866958DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative flavin-containing monooxygenase 2 (FMO2) CSB-YP866959DOA
CSB-EP866959DOA
CSB-BP866959DOA
CSB-MP866959DOA
CSB-EP866959DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box protein At5g60560 (At5g60560) CSB-YP866960DOA
CSB-EP866960DOA
CSB-BP866960DOA
CSB-MP866960DOA
CSB-EP866960DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Chloroplastic group IIB intron splicing facilitator CRS2-A, chloroplastic (CRS2A) CSB-YP866961DOA
CSB-EP866961DOA
CSB-BP866961DOA
CSB-MP866961DOA
CSB-EP866961DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor bHLH99 (BHLH99) CSB-YP866962DOA
CSB-EP866962DOA
CSB-BP866962DOA
CSB-MP866962DOA
CSB-EP866962DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Actin-related protein 8 (ARP8) CSB-YP866963DOA
CSB-EP866963DOA
CSB-BP866963DOA
CSB-MP866963DOA
CSB-EP866963DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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