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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana F-box/FBD/LRR-repeat protein At5g53840 (At5g53840) CSB-YP872220DOA
CSB-EP872220DOA
CSB-BP872220DOA
CSB-MP872220DOA
CSB-EP872220DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana High-affinity nitrate transporter 3.1 (NRT3.1), partial CSB-YP872221DOA1
CSB-EP872221DOA1
CSB-BP872221DOA1
CSB-MP872221DOA1
CSB-EP872221DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Beta-D-xylosidase 1 (BXL1), partial CSB-YP872222DOA
CSB-EP872222DOA
CSB-BP872222DOA
CSB-MP872222DOA
CSB-EP872222DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Serine carboxypeptidase-like 42 (SCPL42) CSB-YP872223DOA
CSB-EP872223DOA
CSB-BP872223DOA
CSB-MP872223DOA
CSB-EP872223DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 2S seed storage protein 5 (SESA5) CSB-YP872224DOA
CSB-EP872224DOA
CSB-BP872224DOA
CSB-MP872224DOA
CSB-EP872224DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box protein At5g41490 (At5g41490) CSB-YP872225DOA
CSB-EP872225DOA
CSB-BP872225DOA
CSB-MP872225DOA
CSB-EP872225DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana AP2-like ethylene-responsive transcription factor TOE3 (TOE3) CSB-YP872226DOA
CSB-EP872226DOA
CSB-BP872226DOA
CSB-MP872226DOA
CSB-EP872226DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative rRNA 2'-O-methyltransferase fibrillarin 3 (FIB3) CSB-YP872227DOA
CSB-EP872227DOA
CSB-BP872227DOA
CSB-MP872227DOA
CSB-EP872227DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein PHLOEM PROTEIN 2-LIKE A6 (PP2A6) CSB-YP872228DOA
CSB-EP872228DOA
CSB-BP872228DOA
CSB-MP872228DOA
CSB-EP872228DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana B3 domain-containing protein At5g57720 (At5g57720) CSB-YP872229DOA
CSB-EP872229DOA
CSB-BP872229DOA
CSB-MP872229DOA
CSB-EP872229DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable leucine-rich repeat receptor-like protein kinase At5g05160 (At5g05160), partial CSB-YP872230DOA1
CSB-EP872230DOA1
CSB-BP872230DOA1
CSB-MP872230DOA1
CSB-EP872230DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box protein At5g65850 (At5g65850) CSB-YP872231DOA
CSB-EP872231DOA
CSB-BP872231DOA
CSB-MP872231DOA
CSB-EP872231DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box protein At5g37040 (At5g37040) CSB-YP872232DOA
CSB-EP872232DOA
CSB-BP872232DOA
CSB-MP872232DOA
CSB-EP872232DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 26S proteasome non-ATPase regulatory subunit RPN12B (RPN12B) CSB-YP872233DOA
CSB-EP872233DOA
CSB-BP872233DOA
CSB-MP872233DOA
CSB-EP872233DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana FBD-associated F-box protein At5g44490 (At5g44490) CSB-YP872234DOA
CSB-EP872234DOA
CSB-BP872234DOA
CSB-MP872234DOA
CSB-EP872234DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cytochrome P450 705A5 (CYP705A5), partial CSB-YP872235DOA1
CSB-EP872235DOA1
CSB-BP872235DOA1
CSB-MP872235DOA1
CSB-EP872235DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/kelch-repeat protein At5g49000 (At5g49000) CSB-YP872236DOA
CSB-EP872236DOA
CSB-BP872236DOA
CSB-MP872236DOA
CSB-EP872236DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana UDP-glycosyltransferase 76C4 (UGT76C4) CSB-YP872237DOA
CSB-EP872237DOA
CSB-BP872237DOA
CSB-MP872237DOA
CSB-EP872237DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ABC transporter F family member 2 (ABCF2), partial CSB-YP872238DOA
CSB-EP872238DOA
CSB-BP872238DOA
CSB-MP872238DOA
CSB-EP872238DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable receptor-like protein kinase At5g39020 (At5g39020), partial CSB-YP872239DOA
CSB-EP872239DOA
CSB-BP872239DOA
CSB-MP872239DOA
CSB-EP872239DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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