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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex 1, mitochondrial (At5g55070) CSB-YP872280DOA
CSB-EP872280DOA
CSB-BP872280DOA
CSB-MP872280DOA
CSB-EP872280DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ABC transporter A family member 12 (ABCA12), partial CSB-YP872281DOA
CSB-EP872281DOA
CSB-BP872281DOA
CSB-MP872281DOA
CSB-EP872281DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box/kelch-repeat protein At5g24040 (At5g24040) CSB-YP872282DOA
CSB-EP872282DOA
CSB-BP872282DOA
CSB-MP872282DOA
CSB-EP872282DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial (At5g52840) CSB-YP872283DOA
CSB-EP872283DOA
CSB-BP872283DOA
CSB-MP872283DOA
CSB-EP872283DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At5g39350 (PCMP-E16), partial CSB-YP872284DOA
CSB-EP872284DOA
CSB-BP872284DOA
CSB-MP872284DOA
CSB-EP872284DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable peptide/nitrate transporter At5g62730 (At5g62730), partial CSB-YP872285DOA
CSB-EP872285DOA
CSB-BP872285DOA
CSB-MP872285DOA
CSB-EP872285DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Inactive beta-amylase 4, chloroplastic (BAM4) CSB-YP872286DOA
CSB-EP872286DOA
CSB-BP872286DOA
CSB-MP872286DOA
CSB-EP872286DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative FBD-associated F-box protein At5g56410 (At5g56410), partial CSB-YP872287DOA
CSB-EP872287DOA
CSB-BP872287DOA
CSB-MP872287DOA
CSB-EP872287DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Peroxisome biogenesis protein 5 (PEX5), partial CSB-YP872288DOA
CSB-EP872288DOA
CSB-BP872288DOA
CSB-MP872288DOA
CSB-EP872288DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable inactive receptor kinase At5g16590 (At5g16590), partial CSB-YP872289DOA1
CSB-EP872289DOA1
CSB-BP872289DOA1
CSB-MP872289DOA1
CSB-EP872289DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Root phototropism protein 3 (RPT3), partial CSB-YP872290DOA
CSB-EP872290DOA
CSB-BP872290DOA
CSB-MP872290DOA
CSB-EP872290DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Phosphatidylinositol 4-kinase beta 1 (PI4KBETA1), partial CSB-YP872291DOA
CSB-EP872291DOA
CSB-BP872291DOA
CSB-MP872291DOA
CSB-EP872291DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Peroxidase 60 (PER60) CSB-YP872292DOA
CSB-EP872292DOA
CSB-BP872292DOA
CSB-MP872292DOA
CSB-EP872292DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box protein At5g14160 (At5g14160) CSB-YP872293DOA
CSB-EP872293DOA
CSB-BP872293DOA
CSB-MP872293DOA
CSB-EP872293DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative GEM-like protein 8 (At5g23370) CSB-YP872294DOA
CSB-EP872294DOA
CSB-BP872294DOA
CSB-MP872294DOA
CSB-EP872294DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Sugar transport protein 11 (STP11), partial CSB-YP872295DOA
CSB-EP872295DOA
CSB-BP872295DOA
CSB-MP872295DOA
CSB-EP872295DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Amino acid permease 4 (AAP4), partial CSB-YP872296DOA
CSB-EP872296DOA
CSB-BP872296DOA
CSB-MP872296DOA
CSB-EP872296DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Homeobox-leucine zipper protein ATHB-52 (ATHB-52) CSB-YP872297DOA
CSB-EP872297DOA
CSB-BP872297DOA
CSB-MP872297DOA
CSB-EP872297DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor GLABRA 3 (GL3), partial CSB-YP872298DOA
CSB-EP872298DOA
CSB-BP872298DOA
CSB-MP872298DOA
CSB-EP872298DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Mitochondrial import receptor subunit TOM22 homolog 2 (TOM22-2), partial CSB-YP872299DOA
CSB-EP872299DOA
CSB-BP872299DOA
CSB-MP872299DOA
CSB-EP872299DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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