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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pisum sativum Beta-amyrin synthase (OSCPSY), partial CSB-YP873324EWE
CSB-EP873324EWE
CSB-BP873324EWE
CSB-MP873324EWE
CSB-EP873324EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cysteine-rich repeat secretory protein 34 (CRRSP34) CSB-YP873325DOA
CSB-EP873325DOA
CSB-BP873325DOA
CSB-MP873325DOA
CSB-EP873325DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative cysteine-rich repeat secretory protein 27 (CRRSP27) CSB-YP873326DOA
CSB-EP873326DOA
CSB-BP873326DOA
CSB-MP873326DOA
CSB-EP873326DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cysteine-rich repeat secretory protein 18 (CRRSP18) CSB-YP873327DOA
CSB-EP873327DOA
CSB-BP873327DOA
CSB-MP873327DOA
CSB-EP873327DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic (NCED6), partial CSB-YP873328DOA
CSB-EP873328DOA
CSB-BP873328DOA
CSB-MP873328DOA
CSB-EP873328DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative geranylgeranyl pyrophosphate synthase 8, chloroplastic (At3g14510) CSB-YP873329DOA
CSB-EP873329DOA
CSB-BP873329DOA
CSB-MP873329DOA
CSB-EP873329DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At3g26540 (PCMP-A5), partial CSB-YP873330DOA
CSB-EP873330DOA
CSB-BP873330DOA
CSB-MP873330DOA
CSB-EP873330DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pumilio homolog 24 (APUM24), partial CSB-YP873331DOA
CSB-EP873331DOA
CSB-BP873331DOA
CSB-MP873331DOA
CSB-EP873331DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Allene oxide cyclase 1, chloroplastic (AOC1) CSB-YP873332DOA
CSB-EP873332DOA
CSB-BP873332DOA
CSB-MP873332DOA
CSB-EP873332DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At5g46580, chloroplastic (At5g46580), partial CSB-YP873333DOA
CSB-EP873333DOA
CSB-BP873333DOA
CSB-MP873333DOA
CSB-EP873333DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box protein At3g18340 (At3g18340) CSB-YP873334DOA
CSB-EP873334DOA
CSB-BP873334DOA
CSB-MP873334DOA
CSB-EP873334DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/kelch-repeat protein At3g18720 (At3g18720) CSB-YP873335DOA
CSB-EP873335DOA
CSB-BP873335DOA
CSB-MP873335DOA
CSB-EP873335DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Serine/threonine-protein kinase OXI1 (OXI1) CSB-YP873336DOA
CSB-EP873336DOA
CSB-BP873336DOA
CSB-MP873336DOA
CSB-EP873336DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At3g18020 (At3g18020), partial CSB-YP873337DOA
CSB-EP873337DOA
CSB-BP873337DOA
CSB-MP873337DOA
CSB-EP873337DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Calmodulin-binding transcription activator 6 (CMTA6), partial CSB-YP873338DOA
CSB-EP873338DOA
CSB-BP873338DOA
CSB-MP873338DOA
CSB-EP873338DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein LURP-one-related 13 (At3g16900) CSB-YP873339DOA
CSB-EP873339DOA
CSB-BP873339DOA
CSB-MP873339DOA
CSB-EP873339DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana tRNA-splicing endonuclease subunit Sen2-2 (SEN2) CSB-YP873340DOA
CSB-EP873340DOA
CSB-BP873340DOA
CSB-MP873340DOA
CSB-EP873340DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Actin-related protein 9 (ARP9), partial CSB-YP873341DOA
CSB-EP873341DOA
CSB-BP873341DOA
CSB-MP873341DOA
CSB-EP873341DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana RING-H2 finger protein ATL16 (ATL16), partial CSB-YP873342DOA1
CSB-EP873342DOA1
CSB-BP873342DOA1
CSB-MP873342DOA1
CSB-EP873342DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Sugar transport protein 10 (STP10), partial CSB-YP873343DOA
CSB-EP873343DOA
CSB-BP873343DOA
CSB-MP873343DOA
CSB-EP873343DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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