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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana F-box/kelch-repeat protein At3g16740 (At3g16740) CSB-YP873364DOA
CSB-EP873364DOA
CSB-BP873364DOA
CSB-MP873364DOA
CSB-EP873364DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box protein At3g16590 (At3g16590) CSB-YP873365DOA
CSB-EP873365DOA
CSB-BP873365DOA
CSB-MP873365DOA
CSB-EP873365DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box/kelch-repeat protein At3g17280 (At3g17280) CSB-YP873366DOA
CSB-EP873366DOA
CSB-BP873366DOA
CSB-MP873366DOA
CSB-EP873366DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable protein phosphatase 2C 43 (At3g17250) CSB-YP873367DOA
CSB-EP873367DOA
CSB-BP873367DOA
CSB-MP873367DOA
CSB-EP873367DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana DEAD-box ATP-dependent RNA helicase 9 (RH9), partial CSB-YP873368DOA
CSB-EP873368DOA
CSB-BP873368DOA
CSB-MP873368DOA
CSB-EP873368DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box/LRR-repeat protein At3g18150 (At3g18150) CSB-YP873369DOA
CSB-EP873369DOA
CSB-BP873369DOA
CSB-MP873369DOA
CSB-EP873369DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Heat stress transcription factor C-1 (HSFC1) CSB-YP873370DOA
CSB-EP873370DOA
CSB-BP873370DOA
CSB-MP873370DOA
CSB-EP873370DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Glutamate receptor 1.2 (GLR1.2), partial CSB-YP873371DOA
CSB-EP873371DOA
CSB-BP873371DOA
CSB-MP873371DOA
CSB-EP873371DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At5g62370 (At5g62370), partial CSB-YP873372DOA
CSB-EP873372DOA
CSB-BP873372DOA
CSB-MP873372DOA
CSB-EP873372DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At5g57250, mitochondrial (At5g57250), partial CSB-YP873373DOA
CSB-EP873373DOA
CSB-BP873373DOA
CSB-MP873373DOA
CSB-EP873373DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At3g21470 (PCMP-E29), partial CSB-YP873374DOA
CSB-EP873374DOA
CSB-BP873374DOA
CSB-MP873374DOA
CSB-EP873374DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box protein At5g60060 (At5g60060) CSB-YP873375DOA
CSB-EP873375DOA
CSB-BP873375DOA
CSB-MP873375DOA
CSB-EP873375DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ubiquitin-like protein ATG12B (ATG12B) CSB-YP873376DOA
CSB-EP873376DOA
CSB-BP873376DOA
CSB-MP873376DOA
CSB-EP873376DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ABC transporter B family member 25, mitochondrial (ABCB25), partial CSB-YP873377DOA1
CSB-EP873377DOA1
CSB-BP873377DOA1
CSB-MP873377DOA1
CSB-EP873377DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Vesicle transport v-SNARE 13 (VTI13), partial CSB-YP873378DOA
CSB-EP873378DOA
CSB-BP873378DOA
CSB-MP873378DOA
CSB-EP873378DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana UDP-glycosyltransferase 72E2 (UGT72E2) CSB-YP873379DOA
CSB-MP873379DOA
CSB-EP873379DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Vesicle-associated protein 2-1 (PVA21), partial CSB-YP873380DOA
CSB-EP873380DOA
CSB-BP873380DOA
CSB-MP873380DOA
CSB-EP873380DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Heat stress transcription factor A-9 (HSFA9) CSB-YP873381DOA
CSB-EP873381DOA
CSB-BP873381DOA
CSB-MP873381DOA
CSB-EP873381DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Tirucalladienol synthase (PEN3), partial CSB-YP873382DOA
CSB-EP873382DOA
CSB-BP873382DOA
CSB-MP873382DOA
CSB-EP873382DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box protein At3g23420 (At3g23420) CSB-YP873383DOA
CSB-EP873383DOA
CSB-BP873383DOA
CSB-MP873383DOA
CSB-EP873383DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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