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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Arabinogalactan peptide 15 (AGP15) CSB-YP873404DOA
CSB-EP873404DOA
CSB-BP873404DOA
CSB-MP873404DOA
CSB-EP873404DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable methyltransferase PMT22 (At3g56080), partial CSB-YP873405DOA
CSB-EP873405DOA
CSB-BP873405DOA
CSB-MP873405DOA
CSB-EP873405DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana CBL-interacting serine/threonine-protein kinase 2 (CIPK2) CSB-YP873406DOA
CSB-EP873406DOA
CSB-BP873406DOA
CSB-MP873406DOA
CSB-EP873406DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein TRANSPARENT TESTA 12 (TT12), partial CSB-YP873407DOA
CSB-EP873407DOA
CSB-BP873407DOA
CSB-MP873407DOA
CSB-EP873407DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana GEM-like protein 5 (At5g13200) CSB-YP873408DOA
CSB-EP873408DOA
CSB-BP873408DOA
CSB-MP873408DOA
CSB-EP873408DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box/kelch-repeat protein At5g02980 (At5g02980) CSB-YP873409DOA
CSB-EP873409DOA
CSB-BP873409DOA
CSB-MP873409DOA
CSB-EP873409DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 40S ribosomal protein S17-4 (RPS17D) CSB-YP873410DOA
CSB-EP873410DOA
CSB-BP873410DOA
CSB-MP873410DOA
CSB-EP873410DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Dof zinc finger protein DOF5.1 (DOF5.1) CSB-YP873411DOA
CSB-EP873411DOA
CSB-BP873411DOA
CSB-MP873411DOA
CSB-EP873411DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ABC transporter I family member 20 (ABCI20) CSB-YP873412DOA
CSB-EP873412DOA
CSB-BP873412DOA
CSB-MP873412DOA
CSB-EP873412DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ABC transporter B family member 29, chloroplastic (ABCB29), partial CSB-YP873413DOA1
CSB-EP873413DOA1
CSB-BP873413DOA1
CSB-MP873413DOA1
CSB-EP873413DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 60S ribosomal protein L7a-2 (RPL7AB) CSB-YP873414DOA
CSB-EP873414DOA
CSB-BP873414DOA
CSB-MP873414DOA
CSB-EP873414DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative cyclin-D7-1 (CYCD7-1) CSB-YP873415DOA
CSB-EP873415DOA
CSB-BP873415DOA
CSB-MP873415DOA
CSB-EP873415DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At3g62470, mitochondrial (At3g62470) CSB-YP873416DOA
CSB-EP873416DOA
CSB-BP873416DOA
CSB-MP873416DOA
CSB-EP873416DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 1,4-alpha-glucan-branching enzyme 2-2, chloroplastic/amyloplastic (SBE2.2), partial CSB-YP873417DOA
CSB-EP873417DOA
CSB-BP873417DOA
CSB-MP873417DOA
CSB-EP873417DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cysteine-rich receptor-like protein kinase 4 (CRK4), partial CSB-YP873418DOA
CSB-EP873418DOA
CSB-BP873418DOA
CSB-MP873418DOA
CSB-EP873418DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana U-box domain-containing protein 16 (PUB16), partial CSB-YP873419DOA
CSB-EP873419DOA
CSB-BP873419DOA
CSB-MP873419DOA
CSB-EP873419DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative FBD-associated F-box protein At3g60710 (At3g60710) CSB-YP873420DOA
CSB-EP873420DOA
CSB-BP873420DOA
CSB-MP873420DOA
CSB-EP873420DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized protein At5g01610 (At5g01610) CSB-YP873421DOA
CSB-EP873421DOA
CSB-BP873421DOA
CSB-MP873421DOA
CSB-EP873421DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative GEM-like protein 3 (At4g40100) CSB-YP873422DOA
CSB-EP873422DOA
CSB-BP873422DOA
CSB-MP873422DOA
CSB-EP873422DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Glucan endo-1,3-beta-glucosidase 5 (At4g31140) CSB-YP873423DOA
CSB-EP873423DOA
CSB-BP873423DOA
CSB-MP873423DOA
CSB-EP873423DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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