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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Corynebacterium glutamicum S-adenosylmethionine synthase (metK) CSB-YP873003DWY
CSB-EP873003DWY
CSB-BP873003DWY
CSB-MP873003DWY
CSB-EP873003DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans 50S ribosomal protein L9 (rplI) CSB-YP873004BQS
CSB-EP873004BQS
CSB-BP873004BQS
CSB-MP873004BQS
CSB-EP873004BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans L-lactate permease (lctP), partial CSB-YP873005BQS
CSB-EP873005BQS
CSB-BP873005BQS
CSB-MP873005BQS
CSB-EP873005BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Malonyl-CoA O-methyltransferase BioC (bioC) CSB-YP873006BQS
CSB-EP873006BQS
CSB-BP873006BQS
CSB-MP873006BQS
CSB-EP873006BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Agmatinase (speB) CSB-YP873007BQS
CSB-BP873007BQS
CSB-MP873007BQS
CSB-EP873007BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans UPF0059 membrane protein BH3770 (BH3770), partial CSB-YP873008BQS1
CSB-EP873008BQS1
CSB-BP873008BQS1
CSB-MP873008BQS1
CSB-EP873008BQS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Pyrrolidone-carboxylate peptidase (pcp) CSB-YP873009BQS
CSB-EP873009BQS
CSB-BP873009BQS
CSB-MP873009BQS
CSB-EP873009BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Protein translocase subunit SecA (secA), partial CSB-YP873010BQS
CSB-EP873010BQS
CSB-BP873010BQS
CSB-MP873010BQS
CSB-EP873010BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP873011BQS
CSB-EP873011BQS
CSB-BP873011BQS
CSB-MP873011BQS
CSB-EP873011BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans ATP-dependent Clp protease proteolytic subunit 1 (clpP1) CSB-YP873012BQS
CSB-EP873012BQS
CSB-BP873012BQS
CSB-MP873012BQS
CSB-EP873012BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Probable cysteine desulfurase (csd) CSB-YP873013BQS
CSB-EP873013BQS
CSB-BP873013BQS
CSB-MP873013BQS
CSB-EP873013BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Uncharacterized protein BH3399 (BH3399) CSB-YP873014BQS
CSB-EP873014BQS
CSB-BP873014BQS
CSB-MP873014BQS
CSB-EP873014BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP873015BQS
CSB-EP873015BQS
CSB-BP873015BQS
CSB-MP873015BQS
CSB-EP873015BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Probable transcriptional regulatory protein BH3259 (BH3259) CSB-YP873016BQS
CSB-EP873016BQS
CSB-BP873016BQS
CSB-MP873016BQS
CSB-EP873016BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Probable tRNA sulfurtransferase (thiI) CSB-YP873017BQS
CSB-EP873017BQS
CSB-BP873017BQS
CSB-MP873017BQS
CSB-EP873017BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Acetate kinase (ackA) CSB-YP873018BQS
CSB-EP873018BQS
CSB-BP873018BQS
CSB-MP873018BQS
CSB-EP873018BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP873019BQS
CSB-EP873019BQS
CSB-BP873019BQS
CSB-MP873019BQS
CSB-EP873019BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP873020BQS
CSB-EP873020BQS
CSB-BP873020BQS
CSB-MP873020BQS
CSB-EP873020BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Protein ArsC (arsC) CSB-YP873021BQS
CSB-EP873021BQS
CSB-BP873021BQS
CSB-MP873021BQS
CSB-EP873021BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Oligo-1,6-glucosidase (malL), partial CSB-YP873022BQS
CSB-EP873022BQS
CSB-BP873022BQS
CSB-MP873022BQS
CSB-EP873022BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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