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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana PRA1 family protein F3 (PRA1F3), partial CSB-YP881734DOA1
CSB-EP881734DOA1
CSB-BP881734DOA1
CSB-MP881734DOA1
CSB-EP881734DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/kelch-repeat protein At3g13680 (At3g13680) CSB-YP881735DOA
CSB-EP881735DOA
CSB-BP881735DOA
CSB-MP881735DOA
CSB-EP881735DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana NifU-like protein 4, mitochondrial (NIFU4) CSB-YP881736DOA
CSB-EP881736DOA
CSB-BP881736DOA
CSB-MP881736DOA
CSB-EP881736DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative vacuolar protein sorting-associated protein 22 homolog 2 (VP22-2) CSB-YP881737DOA
CSB-EP881737DOA
CSB-BP881737DOA
CSB-MP881737DOA
CSB-EP881737DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana PHD finger protein ING1 (ING1) CSB-YP881738DOA
CSB-EP881738DOA
CSB-BP881738DOA
CSB-MP881738DOA
CSB-EP881738DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/kelch-repeat protein At3g23880 (At3g23880) CSB-YP881739DOA
CSB-EP881739DOA
CSB-BP881739DOA
CSB-MP881739DOA
CSB-EP881739DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box protein At3g18980 (At3g18980) CSB-YP881740DOA
CSB-EP881740DOA
CSB-BP881740DOA
CSB-MP881740DOA
CSB-EP881740DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana UPF0496 protein At3g19250 (At3g19250), partial CSB-YP881741DOA1
CSB-EP881741DOA1
CSB-BP881741DOA1
CSB-MP881741DOA1
CSB-EP881741DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable beta-D-xylosidase 5 (BXL5), partial CSB-YP881742DOA
CSB-EP881742DOA
CSB-BP881742DOA
CSB-MP881742DOA
CSB-EP881742DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Purple acid phosphatase 18 (PAP18) CSB-YP881743DOA
CSB-EP881743DOA
CSB-BP881743DOA
CSB-MP881743DOA
CSB-EP881743DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box protein At3g20030 (At3g20030) CSB-YP881744DOA
CSB-EP881744DOA
CSB-BP881744DOA
CSB-MP881744DOA
CSB-EP881744DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative pumilio homolog 25 (APUM25) CSB-YP881745DOA
CSB-EP881745DOA
CSB-BP881745DOA
CSB-MP881745DOA
CSB-EP881745DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable acyl-activating enzyme 16, chloroplastic (AAE16), partial CSB-YP881746DOA
CSB-EP881746DOA
CSB-BP881746DOA
CSB-MP881746DOA
CSB-EP881746DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ras-related protein RABA1g (RABA1G) CSB-YP881747DOA
CSB-EP881747DOA
CSB-BP881747DOA
CSB-MP881747DOA
CSB-EP881747DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana DNA repair endonuclease UVH1 (UVH1), partial CSB-YP881748DOA
CSB-EP881748DOA
CSB-BP881748DOA
CSB-MP881748DOA
CSB-EP881748DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Retinoblastoma-related protein 1 (RBR1), partial CSB-YP881749DOA
CSB-EP881749DOA
CSB-BP881749DOA
CSB-MP881749DOA
CSB-EP881749DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii Cytochrome b6-f complex subunit petO, chloroplastic (PETO), partial CSB-YP881750DSJ1
CSB-EP881750DSJ1
CSB-BP881750DSJ1
CSB-MP881750DSJ1
CSB-EP881750DSJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana UPF0725 protein EMB2204 (EMB2204) CSB-YP881751DOA
CSB-EP881751DOA
CSB-BP881751DOA
CSB-MP881751DOA
CSB-EP881751DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana RING-H2 finger protein ATL80 (ATL80), partial CSB-YP881752DOA1
CSB-EP881752DOA1
CSB-BP881752DOA1
CSB-MP881752DOA1
CSB-EP881752DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box/kelch-repeat protein At1g20790 (At1g20790) CSB-YP881753DOA
CSB-EP881753DOA
CSB-BP881753DOA
CSB-MP881753DOA
CSB-EP881753DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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