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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana Putative F-box protein At1g12855 (At1g12855) CSB-YP881774DOA
CSB-EP881774DOA
CSB-BP881774DOA
CSB-MP881774DOA
CSB-EP881774DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At1g62914, mitochondrial (At1g62914), partial CSB-YP881775DOA
CSB-EP881775DOA
CSB-BP881775DOA
CSB-MP881775DOA
CSB-EP881775DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Dynamin-2B (DRP2B), partial CSB-YP881776DOA
CSB-EP881776DOA
CSB-BP881776DOA
CSB-MP881776DOA
CSB-EP881776DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Heme oxygenase 4, chloroplastic (HO4) CSB-YP881777DOA
CSB-EP881777DOA
CSB-BP881777DOA
CSB-MP881777DOA
CSB-EP881777DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box/kelch-repeat protein At1g32430 (At1g32430) CSB-YP881778DOA
CSB-EP881778DOA
CSB-BP881778DOA
CSB-MP881778DOA
CSB-EP881778DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable calcium-binding protein CML17 (CML17) CSB-YP881779DOA
CSB-EP881779DOA
CSB-BP881779DOA
CSB-MP881779DOA
CSB-EP881779DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable 26S proteasome non-ATPase regulatory subunit 3b (At1g75990) CSB-YP881780DOA
CSB-EP881780DOA
CSB-BP881780DOA
CSB-MP881780DOA
CSB-EP881780DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative HVA22-like protein g (HVA22G) CSB-YP881781DOA
CSB-EP881781DOA
CSB-BP881781DOA
CSB-MP881781DOA
CSB-EP881781DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable protein phosphatase 2C 1 (PPC6-6) CSB-YP881782DOA
CSB-EP881782DOA
CSB-BP881782DOA
CSB-MP881782DOA
CSB-EP881782DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana UPF0725 protein At1g23950 (At1g23950) CSB-YP881783DOA
CSB-EP881783DOA
CSB-BP881783DOA
CSB-MP881783DOA
CSB-EP881783DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana GATA transcription factor 25 (GATA25) CSB-YP881784DOA
CSB-EP881784DOA
CSB-BP881784DOA
CSB-MP881784DOA
CSB-EP881784DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative cysteine-rich repeat secretory protein 24 (CRRSP24) CSB-YP881785DOA
CSB-EP881785DOA
CSB-BP881785DOA
CSB-MP881785DOA
CSB-EP881785DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative cysteine-rich repeat secretory protein 20 (CRRSP20) CSB-YP881786DOA
CSB-EP881786DOA
CSB-BP881786DOA
CSB-MP881786DOA
CSB-EP881786DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable sugar phosphate/phosphate translocator At3g17430 (At3g17430), partial CSB-YP881787DOA
CSB-EP881787DOA
CSB-BP881787DOA
CSB-MP881787DOA
CSB-EP881787DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Terpenoid synthase 17 (TPS17), partial CSB-YP881788DOA
CSB-EP881788DOA
CSB-BP881788DOA
CSB-MP881788DOA
CSB-EP881788DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box protein At3g13830 (At3g13830) CSB-YP881789DOA
CSB-EP881789DOA
CSB-BP881789DOA
CSB-MP881789DOA
CSB-EP881789DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Beta-myrcene/ (E)-beta-ocimene synthase 2, chloroplastic (TPS24), partial CSB-YP881790DOA
CSB-EP881790DOA
CSB-BP881790DOA
CSB-MP881790DOA
CSB-EP881790DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana UDP-glycosyltransferase 76E9 (UGT76E9) CSB-YP881791DOA
CSB-EP881791DOA
CSB-BP881791DOA
CSB-MP881791DOA
CSB-EP881791DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein PLANT CADMIUM RESISTANCE 7 (PCR7), partial CSB-YP881792DOA1
CSB-EP881792DOA1
CSB-BP881792DOA1
CSB-MP881792DOA1
CSB-EP881792DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable E3 ubiquitin protein ligase DRIPH (At3g23060) CSB-YP881793DOA
CSB-EP881793DOA
CSB-BP881793DOA
CSB-MP881793DOA
CSB-EP881793DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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