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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Protein TIC 21, chloroplastic (TIC21), partial CSB-YP882859DOA1
CSB-EP882859DOA1
CSB-BP882859DOA1
CSB-MP882859DOA1
CSB-EP882859DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable F-box protein At1g65740 (At1g65740) CSB-YP882860DOA
CSB-EP882860DOA
CSB-BP882860DOA
CSB-MP882860DOA
CSB-EP882860DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/kelch-repeat protein At2g22030 (At2g22030) CSB-YP882861DOA
CSB-EP882861DOA
CSB-BP882861DOA
CSB-MP882861DOA
CSB-EP882861DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box only protein 9 (FBX9) CSB-YP882862DOA
CSB-EP882862DOA
CSB-BP882862DOA
CSB-MP882862DOA
CSB-EP882862DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Arginine decarboxylase 1 (SPE1), partial CSB-YP882863DOA
CSB-EP882863DOA
CSB-BP882863DOA
CSB-MP882863DOA
CSB-EP882863DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana MATE efflux family protein 5 (DTXL1), partial CSB-YP882864DOA
CSB-EP882864DOA
CSB-BP882864DOA
CSB-MP882864DOA
CSB-EP882864DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative B3 domain-containing protein At2g31460 (At2g31460) CSB-YP882865DOA
CSB-EP882865DOA
CSB-BP882865DOA
CSB-MP882865DOA
CSB-EP882865DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor ERF056 (ERF056) CSB-YP882866DOA
CSB-EP882866DOA
CSB-BP882866DOA
CSB-MP882866DOA
CSB-EP882866DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable histone H2A variant 2 (At2g38810) CSB-YP882867DOA
CSB-EP882867DOA
CSB-BP882867DOA
CSB-MP882867DOA
CSB-EP882867DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Presenilin-like protein At2g29900 (At2g29900), partial CSB-YP882868DOA
CSB-EP882868DOA
CSB-BP882868DOA
CSB-MP882868DOA
CSB-EP882868DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Phospholipase A1-Ialpha2, chloroplastic (At2g31690) CSB-YP882869DOA
CSB-EP882869DOA
CSB-BP882869DOA
CSB-MP882869DOA
CSB-EP882869DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Serpin-Z10 (At2g25240) CSB-YP882870DOA
CSB-EP882870DOA
CSB-BP882870DOA
CSB-MP882870DOA
CSB-EP882870DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Nuclear cap-binding protein subunit 1 (ABH1), partial CSB-YP882871DOA
CSB-EP882871DOA
CSB-BP882871DOA
CSB-MP882871DOA
CSB-EP882871DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription activator GLK1 (GLK1) CSB-YP882872DOA
CSB-EP882872DOA
CSB-BP882872DOA
CSB-MP882872DOA
CSB-EP882872DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 3-ketoacyl-CoA synthase 9 (KCS9), partial CSB-YP882873DOA1
CSB-EP882873DOA1
CSB-BP882873DOA1
CSB-MP882873DOA1
CSB-EP882873DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana GDSL esterase/lipase At2g40250 (At2g40250) CSB-YP882874DOA
CSB-EP882874DOA
CSB-BP882874DOA
CSB-MP882874DOA
CSB-EP882874DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cinnamyl alcohol dehydrogenase 3 (CAD3) CSB-YP882875DOA
CSB-EP882875DOA
CSB-BP882875DOA
CSB-MP882875DOA
CSB-EP882875DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box/FBD/LRR-repeat protein At2g05300 (At2g05300) CSB-YP882876DOA
CSB-EP882876DOA
CSB-BP882876DOA
CSB-MP882876DOA
CSB-EP882876DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box protein At2g04810 (At2g04810) CSB-YP882877DOA
CSB-EP882877DOA
CSB-BP882877DOA
CSB-MP882877DOA
CSB-EP882877DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable cyclic nucleotide-gated ion channel 14 (CNGC14), partial CSB-YP882878DOA1
CSB-EP882878DOA1
CSB-BP882878DOA1
CSB-MP882878DOA1
CSB-EP882878DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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