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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Nudix hydrolase 6 (NUDT6) CSB-YP882879DOA
CSB-EP882879DOA
CSB-BP882879DOA
CSB-MP882879DOA
CSB-EP882879DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Serine/threonine-protein phosphatase BSL2 (BSL2), partial CSB-YP882880DOA
CSB-EP882880DOA
CSB-BP882880DOA
CSB-MP882880DOA
CSB-EP882880DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Citrate synthase 3, peroxisomal (CSY3), partial CSB-YP882881DOA
CSB-EP882881DOA
CSB-BP882881DOA
CSB-MP882881DOA
CSB-EP882881DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana UDP-glycosyltransferase 86A1 (UGT86A1) CSB-YP882882DOA
CSB-EP882882DOA
CSB-BP882882DOA
CSB-MP882882DOA
CSB-EP882882DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Zinc finger A20 and AN1 domain-containing stress-associated protein 4 (SAP4) CSB-YP882883DOA
CSB-EP882883DOA
CSB-BP882883DOA
CSB-MP882883DOA
CSB-EP882883DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable fucosyltransferase 5 (FUT5), partial CSB-YP882884DOA
CSB-EP882884DOA
CSB-BP882884DOA
CSB-MP882884DOA
CSB-EP882884DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable fructose-bisphosphate aldolase 1, chloroplastic (FBA1) CSB-YP882885DOA
CSB-EP882885DOA
CSB-BP882885DOA
CSB-MP882885DOA
CSB-EP882885DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana DNA repair protein RAD51 homolog 2 (RAD51B) CSB-YP882886DOA
CSB-EP882886DOA
CSB-BP882886DOA
CSB-MP882886DOA
CSB-EP882886DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Formin-like protein 18 (FH18), partial CSB-YP882887DOA
CSB-EP882887DOA
CSB-BP882887DOA
CSB-MP882887DOA
CSB-EP882887DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein TIC 55, chloroplastic (TIC55), partial CSB-YP882888DOA1
CSB-EP882888DOA1
CSB-BP882888DOA1
CSB-MP882888DOA1
CSB-EP882888DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor ERF120 (ERF120) CSB-YP882889DOA
CSB-EP882889DOA
CSB-BP882889DOA
CSB-MP882889DOA
CSB-EP882889DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Alcohol dehydrogenase-like 2 (At1g22440) CSB-YP882890DOA
CSB-EP882890DOA
CSB-BP882890DOA
CSB-MP882890DOA
CSB-EP882890DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable E3 ubiquitin-protein ligase ARI11 (ARI11), partial CSB-YP882891DOA
CSB-EP882891DOA
CSB-BP882891DOA
CSB-MP882891DOA
CSB-EP882891DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Mitochondrial inner membrane protein OXA1-like (OXA1L), partial CSB-YP882892DOA1
CSB-EP882892DOA1
CSB-BP882892DOA1
CSB-MP882892DOA1
CSB-EP882892DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable cellulose synthase A catalytic subunit 10 [UDP-forming] (CESA10), partial CSB-YP882893DOA
CSB-EP882893DOA
CSB-BP882893DOA
CSB-MP882893DOA
CSB-EP882893DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Auxin response factor 10 (ARF10), partial CSB-YP882894DOA
CSB-EP882894DOA
CSB-BP882894DOA
CSB-MP882894DOA
CSB-EP882894DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Secretory carrier-associated membrane protein 1 (SCAMP1), partial CSB-YP882895DOA1
CSB-EP882895DOA1
CSB-BP882895DOA1
CSB-MP882895DOA1
CSB-EP882895DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable pectinesterase/pectinesterase inhibitor 16 (PME16) CSB-YP882896DOA
CSB-EP882896DOA
CSB-BP882896DOA
CSB-MP882896DOA
CSB-EP882896DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable CCR4-associated factor 1 homolog 7 (CAF1-7) CSB-YP882897DOA
CSB-EP882897DOA
CSB-BP882897DOA
CSB-MP882897DOA
CSB-EP882897DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative glycerol-3-phosphate transporter 5 (At2g13100), partial CSB-YP882898DOA
CSB-EP882898DOA
CSB-BP882898DOA
CSB-MP882898DOA
CSB-EP882898DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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