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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Oligopeptide transporter 1 (OPT1), partial CSB-YP884228DOA
CSB-EP884228DOA
CSB-BP884228DOA
CSB-MP884228DOA
CSB-EP884228DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Zeaxanthin epoxidase, chloroplastic (ZEP), partial CSB-YP884229DOA
CSB-EP884229DOA
CSB-BP884229DOA
CSB-MP884229DOA
CSB-EP884229DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Glucan endo-1,3-beta-glucosidase 9 (At5g58480) CSB-YP884230DOA
CSB-EP884230DOA
CSB-BP884230DOA
CSB-MP884230DOA
CSB-EP884230DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative MO25-like protein At5g47540 (At5g47540) CSB-YP884231DOA
CSB-EP884231DOA
CSB-BP884231DOA
CSB-MP884231DOA
CSB-EP884231DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 23.5 kDa heat shock protein, mitochondrial (HSP23.5) CSB-YP884232DOA
CSB-EP884232DOA
CSB-BP884232DOA
CSB-MP884232DOA
CSB-EP884232DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Nuclear transcription factor Y subunit C-7 (NFYC7) CSB-YP884233DOA
CSB-EP884233DOA
CSB-BP884233DOA
CSB-MP884233DOA
CSB-EP884233DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana KDEL-tailed cysteine endopeptidase CEP1 (CEP1) CSB-YP884234DOA
CSB-EP884234DOA
CSB-BP884234DOA
CSB-MP884234DOA
CSB-EP884234DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor GTE10 (GTE10), partial CSB-YP884235DOA
CSB-EP884235DOA
CSB-BP884235DOA
CSB-MP884235DOA
CSB-EP884235DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Beta-glucosidase 12 (BGLU12) CSB-YP884236DOA
CSB-EP884236DOA
CSB-BP884236DOA
CSB-MP884236DOA
CSB-EP884236DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Chaperone protein dnaJ 49 (ATJ49), partial CSB-YP884237DOA1
CSB-EP884237DOA1
CSB-BP884237DOA1
CSB-MP884237DOA1
CSB-EP884237DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cytochrome P450 71A16 (CYP71A16), partial CSB-YP884238DOA
CSB-EP884238DOA
CSB-BP884238DOA
CSB-MP884238DOA
CSB-EP884238DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor ERF010 (ERF010) CSB-YP884239DOA
CSB-EP884239DOA
CSB-BP884239DOA
CSB-MP884239DOA
CSB-EP884239DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative B3 domain-containing protein At5g58280 (At5g58280) CSB-YP884240DOA
CSB-EP884240DOA
CSB-BP884240DOA
CSB-MP884240DOA
CSB-EP884240DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized protein PHLOEM PROTEIN 2-LIKE A7 (PP2A7) CSB-YP884241DOA
CSB-EP884241DOA
CSB-BP884241DOA
CSB-MP884241DOA
CSB-EP884241DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Zinc finger protein CONSTANS-LIKE 5 (COL5) CSB-YP884242DOA
CSB-EP884242DOA
CSB-BP884242DOA
CSB-MP884242DOA
CSB-EP884242DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 65-kDa microtubule-associated protein 3 (MAP65-3), partial CSB-YP884243DOA
CSB-EP884243DOA
CSB-BP884243DOA
CSB-MP884243DOA
CSB-EP884243DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Polyubiquitin 9 (UBQ9) CSB-YP884244DOA
CSB-EP884244DOA
CSB-BP884244DOA
CSB-MP884244DOA
CSB-EP884244DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein MITOFERRINLIKE 1, chloroplastic (MFL1), partial CSB-YP884245DOA1
CSB-EP884245DOA1
CSB-BP884245DOA1
CSB-MP884245DOA1
CSB-EP884245DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable calcium-binding protein CML43 (CML43) CSB-YP884246DOA
CSB-EP884246DOA
CSB-BP884246DOA
CSB-MP884246DOA
CSB-EP884246DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana BAHD acyltransferase At5g47980 (BAHD1) CSB-YP884247DOA
CSB-EP884247DOA
CSB-BP884247DOA
CSB-MP884247DOA
CSB-EP884247DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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