重組蛋白

重組蛋白質(zhì)是分析生物相互作用的有用工具。在研究實(shí)驗(yàn)室中使用了大量的重組蛋白質(zhì),包括商業(yè)蛋白質(zhì)和在特定研究項(xiàng)目中生成的蛋白質(zhì)。它們?cè)谠S多應(yīng)用中發(fā)揮著關(guān)鍵作用。
如果您正在尋找具有優(yōu)良質(zhì)量(驗(yàn)證的生物活性,較低的內(nèi)毒素水平和高純度)的重組蛋白質(zhì)來(lái)支持您的研究,您來(lái)對(duì)地方了。
經(jīng)過(guò)18年的發(fā)展,華美生物已經(jīng)建立了從原核到真核的五大系統(tǒng),用于表達(dá)重組蛋白質(zhì)。我們擁有一系列蛋白質(zhì)表達(dá)和純化技術(shù),包括基于桿狀病毒外膜蛋白(OMP)的蛋白質(zhì)表達(dá)技術(shù),以及膜蛋白表達(dá)技術(shù)等。
產(chǎn)品特點(diǎn)
- 超過(guò)13,000種重組蛋白覆蓋大多數(shù)基因/靶點(diǎn);
- 5種表達(dá)系統(tǒng),包括大腸桿菌(E.coli)、酵母、桿狀病毒感染的昆蟲(chóng)細(xì)胞、哺乳動(dòng)物細(xì)胞和體外大腸桿菌。而體外大腸桿菌表達(dá)系統(tǒng)是華美生物的獨(dú)特表達(dá)系統(tǒng)。與傳統(tǒng)蛋白表達(dá)系統(tǒng)相比,體外大腸桿菌省略了許多過(guò)程,如質(zhì)粒轉(zhuǎn)化、細(xì)胞培養(yǎng)、收集、破碎和離心等,大大提高了工作效率。
- 多種標(biāo)簽,包括His、GST、Flag、MBP等。
- 廣泛應(yīng)用,包括抗原、細(xì)胞分析、結(jié)合分析/蛋白質(zhì)相互作用、細(xì)胞培養(yǎng)-無(wú)血清培養(yǎng)基、藥物相關(guān)研究、體外酶活性、ELISA標(biāo)準(zhǔn)及其原料、體內(nèi)研究、質(zhì)譜標(biāo)準(zhǔn)、蛋白芯片、SDS-PAGE控制、蛋白質(zhì)結(jié)構(gòu)分析(晶體/電子顯微鏡)。
- 多種物種,包括人類、小鼠、大鼠、兔子、豚鼠、雞、猴子、豬等。
- 無(wú)動(dòng)物副產(chǎn)品的動(dòng)物源性重組蛋白質(zhì)。華美生物已開(kāi)發(fā)出無(wú)動(dòng)物副產(chǎn)品接觸的重組蛋白質(zhì)產(chǎn)品線。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質(zhì),或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的抗體,請(qǐng)查看華美生物蛋白質(zhì)定制服務(wù)。
通過(guò)選擇目標(biāo)名稱的首字母瀏覽產(chǎn)品。
產(chǎn)品名稱 | 貨號(hào) | 規(guī)格 |
---|---|---|
Recombinant Arabidopsis thaliana 2-hydroxyacyl-CoA lyase (HACL), partial | CSB-YP885330DOA CSB-EP885330DOA CSB-BP885330DOA CSB-MP885330DOA CSB-EP885330DOA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Arabidopsis thaliana Calcium-transporting ATPase 8, plasma membrane-type (ACA8), partial | CSB-YP885331DOA CSB-EP885331DOA CSB-BP885331DOA CSB-MP885331DOA CSB-EP885331DOA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At3g53700, chloroplastic (MEE40), partial | CSB-YP885332DOA CSB-EP885332DOA CSB-BP885332DOA CSB-MP885332DOA CSB-EP885332DOA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Arabidopsis thaliana 60S ribosomal protein L12-2 (RPL12B) | CSB-YP885333DOA CSB-EP885333DOA CSB-BP885333DOA CSB-MP885333DOA CSB-EP885333DOA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Arabidopsis thaliana Putative inactive serine/threonine-protein kinase At5g11400 (At5g11400) | CSB-YP885334DOA CSB-EP885334DOA CSB-BP885334DOA CSB-MP885334DOA CSB-EP885334DOA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At5g11310, mitochondrial (At5g11310), partial | CSB-YP885335DOA CSB-EP885335DOA CSB-BP885335DOA CSB-MP885335DOA CSB-EP885335DOA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Arabidopsis thaliana Probable glycosyltransferase At5g11130 (At5g11120/At5g11130), partial | CSB-YP885336DOA CSB-EP885336DOA CSB-BP885336DOA CSB-MP885336DOA CSB-EP885336DOA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Arabidopsis thaliana Probable carboxylesterase 17 (CXE17) | CSB-YP885337DOA CSB-EP885337DOA CSB-BP885337DOA CSB-MP885337DOA CSB-EP885337DOA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Arabidopsis thaliana Probable leucine-rich repeat receptor-like serine/threonine-protein kinase At5g15730 (At5g15730), partial | CSB-YP885338DOA CSB-EP885338DOA CSB-BP885338DOA CSB-MP885338DOA CSB-EP885338DOA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Arabidopsis thaliana Nitrate transporter 1.6 (NRT1.6), partial | CSB-YP885339DOA CSB-EP885339DOA CSB-BP885339DOA CSB-MP885339DOA CSB-EP885339DOA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Arabidopsis thaliana Poly (A)-specific ribonuclease PARN (PARN), partial | CSB-YP885340DOA CSB-EP885340DOA CSB-BP885340DOA CSB-MP885340DOA CSB-EP885340DOA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Oryza sativa subsp. japonica S-adenosylmethionine synthase 3 (METK3) | CSB-YP885341OFG CSB-EP885341OFG CSB-BP885341OFG CSB-MP885341OFG CSB-EP885341OFG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Arabidopsis thaliana Probable rhamnose biosynthetic enzyme 3 (RHM3), partial | CSB-YP885342DOA CSB-EP885342DOA CSB-BP885342DOA CSB-MP885342DOA CSB-EP885342DOA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Arabidopsis thaliana V-type proton ATPase subunit d2 (VHA-D2) | CSB-YP885343DOA CSB-EP885343DOA CSB-BP885343DOA CSB-MP885343DOA CSB-EP885343DOA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Arabidopsis thaliana ABC transporter B family member 15 (ABCB15), partial | CSB-YP885344DOA CSB-EP885344DOA CSB-BP885344DOA CSB-MP885344DOA CSB-EP885344DOA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Arabidopsis thaliana Leucine-rich repeat extensin-like protein 4 (LRX4) | CSB-YP885345DOA CSB-EP885345DOA CSB-BP885345DOA CSB-MP885345DOA CSB-EP885345DOA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Arabidopsis thaliana Putative pentatricopeptide repeat-containing protein At3g18840 (PCMP-E92), partial | CSB-YP885346DOA CSB-EP885346DOA CSB-BP885346DOA CSB-MP885346DOA CSB-EP885346DOA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Arabidopsis thaliana Receptor-like protein kinase 2 (RCH2), partial | CSB-YP885347DOA CSB-EP885347DOA CSB-BP885347DOA CSB-MP885347DOA CSB-EP885347DOA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Arabidopsis thaliana Geranylgeranyl pyrophosphate synthase 12, chloroplastic (At3g32040) | CSB-YP885348DOA CSB-EP885348DOA CSB-BP885348DOA CSB-MP885348DOA CSB-EP885348DOA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Oryza sativa subsp. japonica B3 domain-containing protein Os06g0112300 (Os06g0112300, LOC_Os06g02230) | CSB-YP885349OFG CSB-EP885349OFG CSB-BP885349OFG CSB-MP885349OFG CSB-EP885349OFG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。
第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條:如何選擇合適的表達(dá)載體?
表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。
根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022