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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Cysteine-rich repeat secretory protein 26 (CRRSP26) CSB-YP885410DOA
CSB-EP885410DOA
CSB-BP885410DOA
CSB-MP885410DOA
CSB-EP885410DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative cysteine-rich repeat secretory protein 17 (CRRSP17) CSB-YP885411DOA
CSB-EP885411DOA
CSB-BP885411DOA
CSB-MP885411DOA
CSB-EP885411DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 50S ribosomal protein L3-2, chloroplastic (RPL3B) CSB-YP885412DOA
CSB-EP885412DOA
CSB-BP885412DOA
CSB-MP885412DOA
CSB-EP885412DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Peroxisomal (S)-2-hydroxy-acid oxidase GLO2 (GLO2) CSB-YP885413DOA
CSB-EP885413DOA
CSB-BP885413DOA
CSB-MP885413DOA
CSB-EP885413DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probably inactive leucine-rich repeat receptor-like protein kinase At3g28040 (At3g28040), partial CSB-YP885414DOA
CSB-EP885414DOA
CSB-BP885414DOA
CSB-MP885414DOA
CSB-EP885414DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box protein At3g13820 (At3g13820) CSB-YP885415DOA
CSB-EP885415DOA
CSB-BP885415DOA
CSB-MP885415DOA
CSB-EP885415DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box protein At3g16820 (At3g16820) CSB-YP885416DOA
CSB-EP885416DOA
CSB-BP885416DOA
CSB-MP885416DOA
CSB-EP885416DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Allene oxide cyclase 3, chloroplastic (AOC3) CSB-YP885417DOA
CSB-EP885417DOA
CSB-BP885417DOA
CSB-MP885417DOA
CSB-EP885417DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana UDP-glycosyltransferase 76E7 (UGT76E7) CSB-YP885418DOA
CSB-EP885418DOA
CSB-BP885418DOA
CSB-MP885418DOA
CSB-EP885418DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Carbon catabolite repressor protein 4 homolog 3 (CCR4-3) CSB-YP885419DOA
CSB-EP885419DOA
CSB-BP885419DOA
CSB-MP885419DOA
CSB-EP885419DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At3g23020 (At3g23020), partial CSB-YP885420DOA
CSB-EP885420DOA
CSB-BP885420DOA
CSB-MP885420DOA
CSB-EP885420DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ribonucleoside-diphosphate reductase small chain C (TSO2) CSB-YP885421DOA
CSB-EP885421DOA
CSB-BP885421DOA
CSB-MP885421DOA
CSB-EP885421DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ABC transporter B family member 17 (ABCB17), partial CSB-YP885422DOA
CSB-EP885422DOA
CSB-BP885422DOA
CSB-MP885422DOA
CSB-EP885422DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At5g65570 (PCMP-H47), partial CSB-YP885423DOA
CSB-EP885423DOA
CSB-BP885423DOA
CSB-MP885423DOA
CSB-EP885423DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable pectinesterase 67 (PME67) CSB-YP885424DOA
CSB-EP885424DOA
CSB-BP885424DOA
CSB-MP885424DOA
CSB-EP885424DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Indole-3-acetic acid-amido synthetase GH3.6 (GH3.6), partial CSB-YP885425DOA
CSB-EP885425DOA
CSB-BP885425DOA
CSB-MP885425DOA
CSB-EP885425DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Proteasome subunit alpha type-3 (PAG1) CSB-YP885426OFG
CSB-EP885426OFG
CSB-BP885426OFG
CSB-MP885426OFG
CSB-EP885426OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Peroxisome biogenesis protein 22 (PEX22), partial CSB-YP885427DOA1
CSB-EP885427DOA1
CSB-BP885427DOA1
CSB-MP885427DOA1
CSB-EP885427DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana UDP-glycosyltransferase 71B6 (UGT71B6) CSB-YP885428DOA
CSB-EP885428DOA
CSB-BP885428DOA
CSB-MP885428DOA
CSB-EP885428DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 26S proteasome non-ATPase regulatory subunit 14 (RPN11) CSB-YP885429DOA
CSB-EP885429DOA
CSB-BP885429DOA
CSB-MP885429DOA
CSB-EP885429DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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