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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana V-type proton ATPase subunit H (VHA-H) CSB-YP885470DOA
CSB-EP885470DOA
CSB-BP885470DOA
CSB-MP885470DOA
CSB-EP885470DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Purple acid phosphatase 19 (PAP19) CSB-YP885471DOA
CSB-EP885471DOA
CSB-BP885471DOA
CSB-MP885471DOA
CSB-EP885471DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor bHLH61 (BHLH61) CSB-YP885472DOA
CSB-EP885472DOA
CSB-BP885472DOA
CSB-MP885472DOA
CSB-EP885472DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At5g15340, mitochondrial (PCMP-H91), partial CSB-YP885473DOA
CSB-EP885473DOA
CSB-BP885473DOA
CSB-MP885473DOA
CSB-EP885473DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Purple acid phosphatase 21 (PAP21) CSB-YP885474DOA
CSB-EP885474DOA
CSB-BP885474DOA
CSB-MP885474DOA
CSB-EP885474DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein HIRA (HIRA), partial CSB-YP885475DOA
CSB-EP885475DOA
CSB-BP885475DOA
CSB-MP885475DOA
CSB-EP885475DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box/LRR-repeat protein At3g58880 (At3g58880) CSB-YP885476DOA
CSB-EP885476DOA
CSB-BP885476DOA
CSB-MP885476DOA
CSB-EP885476DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Callose synthase 3 (CALS3), partial CSB-YP885477DOA
CSB-EP885477DOA
CSB-BP885477DOA
CSB-MP885477DOA
CSB-EP885477DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Zinc finger CCCH domain-containing protein 56 (At5g12850), partial CSB-YP885478DOA
CSB-EP885478DOA
CSB-BP885478DOA
CSB-MP885478DOA
CSB-EP885478DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At3g56550 (PCMP-H80), partial CSB-YP885479DOA
CSB-EP885479DOA
CSB-BP885479DOA
CSB-MP885479DOA
CSB-EP885479DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor ERF106 (ERF106) CSB-YP885480DOA
CSB-EP885480DOA
CSB-BP885480DOA
CSB-MP885480DOA
CSB-EP885480DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ATPase 7, plasma membrane-type (AHA7), partial CSB-YP885481DOA
CSB-EP885481DOA
CSB-BP885481DOA
CSB-MP885481DOA
CSB-EP885481DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana NPL4-like protein 1 (At3g63000) CSB-YP885482DOA
CSB-EP885482DOA
CSB-BP885482DOA
CSB-MP885482DOA
CSB-EP885482DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana DEAD-box ATP-dependent RNA helicase 46 (RH46), partial CSB-YP885483DOA
CSB-EP885483DOA
CSB-BP885483DOA
CSB-MP885483DOA
CSB-EP885483DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ferritin-3, chloroplastic (FER3) CSB-YP885484DOA
CSB-EP885484DOA
CSB-BP885484DOA
CSB-MP885484DOA
CSB-EP885484DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Laccase-13 (LAC13), partial CSB-YP885485DOA
CSB-EP885485DOA
CSB-BP885485DOA
CSB-MP885485DOA
CSB-EP885485DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable pectinesterase/pectinesterase inhibitor 35 (PME35) CSB-YP885486DOA
CSB-EP885486DOA
CSB-BP885486DOA
CSB-MP885486DOA
CSB-EP885486DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable cytokinin riboside 5'-monophosphate phosphoribohydrolase LOG6 (LOG6) CSB-YP885487DOA
CSB-EP885487DOA
CSB-BP885487DOA
CSB-MP885487DOA
CSB-EP885487DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box/LRR-repeat protein At5g02930 (At5g02930) CSB-YP885488DOA
CSB-EP885488DOA
CSB-BP885488DOA
CSB-MP885488DOA
CSB-EP885488DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box/LRR-repeat protein At5g02700 (At5g02700) CSB-YP885489DOA
CSB-EP885489DOA
CSB-BP885489DOA
CSB-MP885489DOA
CSB-EP885489DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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