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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Dof zinc finger protein DOF4.1 (DOF4.1) CSB-YP885510DOA
CSB-EP885510DOA
CSB-BP885510DOA
CSB-MP885510DOA
CSB-EP885510DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein NLP9 (NLP9), partial CSB-YP885511DOA
CSB-EP885511DOA
CSB-BP885511DOA
CSB-MP885511DOA
CSB-EP885511DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Nitrate excretion transporter 1 (NAXT1), partial CSB-YP885512DOA
CSB-EP885512DOA
CSB-BP885512DOA
CSB-MP885512DOA
CSB-EP885512DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Regulatory protein NPR6 (NPR6) CSB-YP885513DOA
CSB-EP885513DOA
CSB-BP885513DOA
CSB-MP885513DOA
CSB-EP885513DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box protein At3g62230 (At3g62230) CSB-YP885514DOA
CSB-EP885514DOA
CSB-BP885514DOA
CSB-MP885514DOA
CSB-EP885514DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor TCP16 (TCP16) CSB-YP885515DOA
CSB-EP885515DOA
CSB-BP885515DOA
CSB-MP885515DOA
CSB-EP885515DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana SKP1-like protein 5 (ASK5) CSB-YP885516DOA
CSB-EP885516DOA
CSB-BP885516DOA
CSB-MP885516DOA
CSB-EP885516DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative L-type lectin-domain containing receptor kinase V.8 (LECRK58), partial CSB-YP885517DOA
CSB-EP885517DOA
CSB-BP885517DOA
CSB-MP885517DOA
CSB-EP885517DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Germin-like protein subfamily 2 member 4 (GLP10) CSB-YP885518DOA
CSB-EP885518DOA
CSB-BP885518DOA
CSB-MP885518DOA
CSB-EP885518DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At3g42630 (At3g42630) CSB-YP885519DOA
CSB-BP885519DOA
CSB-MP885519DOA
CSB-EP885519DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At3g61360 (At3g61360) CSB-YP885520DOA
CSB-EP885520DOA
CSB-BP885520DOA
CSB-MP885520DOA
CSB-EP885520DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Carbon catabolite repressor protein 4 homolog 2 (CCR4-2), partial CSB-YP885521DOA
CSB-EP885521DOA
CSB-BP885521DOA
CSB-MP885521DOA
CSB-EP885521DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Bet1-like SNARE 1-1 (BET11), partial CSB-YP885522DOA
CSB-EP885522DOA
CSB-BP885522DOA
CSB-MP885522DOA
CSB-EP885522DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana GDSL esterase/lipase At3g50400 (At3g50400) CSB-YP885523DOA
CSB-EP885523DOA
CSB-BP885523DOA
CSB-MP885523DOA
CSB-EP885523DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Dof zinc finger protein DOF3.6 (DOF3.6) CSB-YP885524DOA
CSB-EP885524DOA
CSB-BP885524DOA
CSB-MP885524DOA
CSB-EP885524DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At3g49740 (PCMP-E84), partial CSB-YP885525DOA
CSB-EP885525DOA
CSB-BP885525DOA
CSB-MP885525DOA
CSB-EP885525DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Membrane steroid-binding protein 2 (MSBP2), partial CSB-YP885526DOA1
CSB-EP885526DOA1
CSB-BP885526DOA1
CSB-MP885526DOA1
CSB-EP885526DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable proteasome inhibitor (At3g53970) CSB-YP885527DOA
CSB-EP885527DOA
CSB-BP885527DOA
CSB-MP885527DOA
CSB-EP885527DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Wall-associated receptor kinase-like 15 (WAKL15), partial CSB-YP885528DOA
CSB-EP885528DOA
CSB-BP885528DOA
CSB-MP885528DOA
CSB-EP885528DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable F-box protein At3g61730 (RMF) CSB-YP885529DOA
CSB-EP885529DOA
CSB-BP885529DOA
CSB-MP885529DOA
CSB-EP885529DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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