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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus halodurans Zinc metalloprotease rasP (rasP), partial CSB-YP885110BQS1
CSB-EP885110BQS1
CSB-BP885110BQS1
CSB-MP885110BQS1
CSB-EP885110BQS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Ribosome-binding factor A (rbfA) CSB-YP885111BQS
CSB-EP885111BQS
CSB-BP885111BQS
CSB-MP885111BQS
CSB-EP885111BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans DNA translocase FtsK (ftsK), partial CSB-YP885112BQS1
CSB-EP885112BQS1
CSB-BP885112BQS1
CSB-MP885112BQS1
CSB-EP885112BQS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans DNA mismatch repair protein mutL (mutL), partial CSB-YP885113BQS
CSB-EP885113BQS
CSB-BP885113BQS
CSB-MP885113BQS
CSB-EP885113BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans 5-deoxy-glucuronate isomerase (iolB) CSB-YP885114BQS
CSB-EP885114BQS
CSB-BP885114BQS
CSB-MP885114BQS
CSB-EP885114BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Methylmalonate semialdehyde dehydrogenase [acylating] (iolA) CSB-YP885115BQS
CSB-EP885115BQS
CSB-BP885115BQS
CSB-MP885115BQS
CSB-EP885115BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Reducing end xylose-releasing exo-oligoxylanase (BH2105) CSB-YP885116BQS
CSB-EP885116BQS
CSB-BP885116BQS
CSB-MP885116BQS
CSB-EP885116BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans L-arabinose isomerase (araA) CSB-YP885117BQS
CSB-EP885117BQS
CSB-BP885117BQS
CSB-MP885117BQS
CSB-EP885117BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Metallothiol transferase fosB (fosB) CSB-YP885118BQS
CSB-EP885118BQS
CSB-BP885118BQS
CSB-MP885118BQS
CSB-EP885118BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans L-threonine dehydratase biosynthetic IlvA (ilvA) CSB-YP885119BQS
CSB-EP885119BQS
CSB-BP885119BQS
CSB-MP885119BQS
CSB-EP885119BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Tryptophan synthase alpha chain (trpA) CSB-YP885120BQS
CSB-EP885120BQS
CSB-BP885120BQS
CSB-MP885120BQS
CSB-EP885120BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Nucleoside diphosphate kinase (ndk) CSB-YP885121BQS
CSB-EP885121BQS
CSB-BP885121BQS
CSB-MP885121BQS
CSB-EP885121BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans GTPase Der (der) CSB-YP885122BQS
CSB-EP885122BQS
CSB-BP885122BQS
CSB-MP885122BQS
CSB-EP885122BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP885123BQS
CSB-EP885123BQS
CSB-BP885123BQS
CSB-MP885123BQS
CSB-EP885123BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Glutamate 5-kinase (proB) CSB-YP885124BQS
CSB-EP885124BQS
CSB-BP885124BQS
CSB-MP885124BQS
CSB-EP885124BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP885125BQS
CSB-EP885125BQS
CSB-BP885125BQS
CSB-MP885125BQS
CSB-EP885125BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Fumarate hydratase class II (fumC) CSB-YP885126BQS
CSB-EP885126BQS
CSB-BP885126BQS
CSB-MP885126BQS
CSB-EP885126BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Polyphosphate kinase (ppk), partial CSB-YP885127BQS
CSB-EP885127BQS
CSB-BP885127BQS
CSB-MP885127BQS
CSB-EP885127BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans UPF0135 protein BH1380 (BH1380) CSB-YP885128BQS
CSB-EP885128BQS
CSB-BP885128BQS
CSB-MP885128BQS
CSB-EP885128BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Chaperone protein DnaK (dnaK), partial CSB-YP885129BQS
CSB-EP885129BQS
CSB-BP885129BQS
CSB-MP885129BQS
CSB-EP885129BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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