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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana UPF0725 protein At3g19520 (At3g19520) CSB-YP888723DOA
CSB-EP888723DOA
CSB-BP888723DOA
CSB-MP888723DOA
CSB-EP888723DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box protein At3g28280 (At3g28280) CSB-YP888724DOA
CSB-EP888724DOA
CSB-BP888724DOA
CSB-MP888724DOA
CSB-EP888724DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Nascent polypeptide-associated complex subunit alpha-like protein 1 (At3g12390) CSB-YP888725DOA
CSB-EP888725DOA
CSB-BP888725DOA
CSB-MP888725DOA
CSB-EP888725DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 60S ribosomal protein L7-4 (RPL7D) CSB-YP888726DOA
CSB-EP888726DOA
CSB-BP888726DOA
CSB-MP888726DOA
CSB-EP888726DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Glycerol-3-phosphate acyltransferase 7 (GPAT7), partial CSB-YP888727DOA1
CSB-EP888727DOA1
CSB-BP888727DOA1
CSB-MP888727DOA1
CSB-EP888727DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/kelch-repeat protein At3g27150 (At3g27150) CSB-YP888728DOA
CSB-EP888728DOA
CSB-BP888728DOA
CSB-MP888728DOA
CSB-EP888728DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana COBRA-like protein 8 (COBL8), partial CSB-YP888729DOA
CSB-EP888729DOA
CSB-BP888729DOA
CSB-MP888729DOA
CSB-EP888729DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At3g22150, chloroplastic (PCMP-E95), partial CSB-YP888730DOA
CSB-EP888730DOA
CSB-BP888730DOA
CSB-MP888730DOA
CSB-EP888730DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana DEAD-box ATP-dependent RNA helicase 51 (RH51), partial CSB-YP888731DOA
CSB-EP888731DOA
CSB-BP888731DOA
CSB-MP888731DOA
CSB-EP888731DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative pentatricopeptide repeat-containing protein At3g15200 (At3g15200), partial CSB-YP888732DOA
CSB-EP888732DOA
CSB-BP888732DOA
CSB-MP888732DOA
CSB-EP888732DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box protein At3g23960 (At3g23960) CSB-YP888733DOA
CSB-EP888733DOA
CSB-BP888733DOA
CSB-MP888733DOA
CSB-EP888733DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box protein At3g29830 (At3g29830) CSB-YP888734DOA
CSB-EP888734DOA
CSB-BP888734DOA
CSB-MP888734DOA
CSB-EP888734DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Constitutive photomorphogenesis protein 10 (COP10) CSB-YP888735DOA
CSB-EP888735DOA
CSB-BP888735DOA
CSB-MP888735DOA
CSB-EP888735DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/FBD/LRR-repeat protein At3g26920 (At3g26920) CSB-YP888736DOA
CSB-EP888736DOA
CSB-BP888736DOA
CSB-MP888736DOA
CSB-EP888736DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cation/calcium exchanger 3 (CCX3), partial CSB-YP888737DOA
CSB-EP888737DOA
CSB-BP888737DOA
CSB-MP888737DOA
CSB-EP888737DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Receptor-like protein kinase HAIKU2 (IKU2), partial CSB-YP888739DOA
CSB-EP888739DOA
CSB-BP888739DOA
CSB-MP888739DOA
CSB-EP888739DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein SPA1-RELATED 3 (SPA3), partial CSB-YP888740DOA
CSB-EP888740DOA
CSB-BP888740DOA
CSB-MP888740DOA
CSB-EP888740DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Proline-rich receptor-like protein kinase PERK2 (PERK2), partial CSB-YP888741DOA
CSB-EP888741DOA
CSB-BP888741DOA
CSB-MP888741DOA
CSB-EP888741DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Kelch repeat-containing protein At3g27220 (At3g27220), partial CSB-YP888742DOA1
CSB-EP888742DOA1
CSB-BP888742DOA1
CSB-MP888742DOA1
CSB-EP888742DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Selenium-binding protein 3 (SBP3) CSB-YP888743DOA
CSB-EP888743DOA
CSB-BP888743DOA
CSB-MP888743DOA
CSB-EP888743DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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