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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Glutathione S-transferase U8 (GSTU8) CSB-YP890407DOA
CSB-EP890407DOA
CSB-BP890407DOA
CSB-MP890407DOA
CSB-EP890407DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana DEMETER-like protein 2 (DML2), partial CSB-YP890408DOA
CSB-EP890408DOA
CSB-BP890408DOA
CSB-MP890408DOA
CSB-EP890408DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Alanine--glyoxylate aminotransferase 2 homolog 3, mitochondrial (At3g08860) CSB-YP890409DOA
CSB-EP890409DOA
CSB-BP890409DOA
CSB-MP890409DOA
CSB-EP890409DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Vacuolar iron transporter homolog 2 (At1g76800), partial CSB-YP890410DOA1
CSB-EP890410DOA1
CSB-BP890410DOA1
CSB-MP890410DOA1
CSB-EP890410DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein disulfide isomerase-like 1-2 (PDIL1-2) CSB-YP890411DOA
CSB-EP890411DOA
CSB-BP890411DOA
CSB-MP890411DOA
CSB-EP890411DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative 60S ribosomal protein L37a-1 (RPL37AB) CSB-YP890412DOA
CSB-EP890412DOA
CSB-BP890412DOA
CSB-MP890412DOA
CSB-EP890412DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable calcium-binding protein CML33 (CML33) CSB-YP890413DOA
CSB-EP890413DOA
CSB-BP890413DOA
CSB-MP890413DOA
CSB-EP890413DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/LRR-repeat protein 12 (FBL12) CSB-YP890414DOA
CSB-EP890414DOA
CSB-BP890414DOA
CSB-MP890414DOA
CSB-EP890414DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor bHLH62 (BHLH62) CSB-YP890415DOA
CSB-EP890415DOA
CSB-BP890415DOA
CSB-MP890415DOA
CSB-EP890415DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Histone-lysine N-methyltransferase SUVR3 (SUVR3) CSB-YP890416DOA
CSB-EP890416DOA
CSB-BP890416DOA
CSB-MP890416DOA
CSB-EP890416DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable pectinesterase/pectinesterase inhibitor 7 (PME7), partial CSB-YP890417DOA
CSB-EP890417DOA
CSB-BP890417DOA
CSB-MP890417DOA
CSB-EP890417DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Glutathione S-transferase F5 (GSTF5) CSB-YP890418DOA
CSB-EP890418DOA
CSB-BP890418DOA
CSB-MP890418DOA
CSB-EP890418DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 40S ribosomal protein S24-1 (RPS24A) CSB-YP890419DOA
CSB-EP890419DOA
CSB-BP890419DOA
CSB-MP890419DOA
CSB-EP890419DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription initiation factor IIB-2 (TFIIB2) CSB-YP890420DOA
CSB-EP890420DOA
CSB-BP890420DOA
CSB-MP890420DOA
CSB-EP890420DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase RGLG1 (RGLG1) CSB-YP890421DOA
CSB-EP890421DOA
CSB-BP890421DOA
CSB-MP890421DOA
CSB-EP890421DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 26S protease regulatory subunit 7 homolog B (RPT1B) CSB-YP890422DOA
CSB-EP890422DOA
CSB-BP890422DOA
CSB-MP890422DOA
CSB-EP890422DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Calcium-dependent protein kinase 30 (CPK30), partial CSB-YP890423DOA
CSB-EP890423DOA
CSB-BP890423DOA
CSB-MP890423DOA
CSB-EP890423DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative boron transporter 5 (BOR5), partial CSB-YP890424DOA
CSB-EP890424DOA
CSB-BP890424DOA
CSB-MP890424DOA
CSB-EP890424DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Threonine synthase 2, chloroplastic (TS2) CSB-YP890425DOA
CSB-EP890425DOA
CSB-BP890425DOA
CSB-MP890425DOA
CSB-EP890425DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana DCC family protein At1g52590, chloroplastic (At1g52590) CSB-YP890426DOA
CSB-EP890426DOA
CSB-BP890426DOA
CSB-MP890426DOA
CSB-EP890426DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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