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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At2g31400, chloroplastic (At2g31400), partial CSB-YP890347DOA
CSB-EP890347DOA
CSB-BP890347DOA
CSB-MP890347DOA
CSB-EP890347DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ras-related protein RABH1d (RABH1D) CSB-YP890348DOA
CSB-EP890348DOA
CSB-BP890348DOA
CSB-MP890348DOA
CSB-EP890348DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At2g17210 (PCMP-E77), partial CSB-YP890349DOA
CSB-EP890349DOA
CSB-BP890349DOA
CSB-MP890349DOA
CSB-EP890349DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Inactive TPR repeat-containing thioredoxin TTL3 (TTL3), partial CSB-YP890350DOA
CSB-EP890350DOA
CSB-BP890350DOA
CSB-MP890350DOA
CSB-EP890350DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana GDSL esterase/lipase At2g31540 (At2g31540) CSB-YP890351DOA
CSB-EP890351DOA
CSB-BP890351DOA
CSB-MP890351DOA
CSB-EP890351DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At2g13600 (PCMP-E76), partial CSB-YP890352DOA
CSB-EP890352DOA
CSB-BP890352DOA
CSB-MP890352DOA
CSB-EP890352DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Purple acid phosphatase 10 (PAP10) CSB-YP890353DOA
CSB-EP890353DOA
CSB-BP890353DOA
CSB-MP890353DOA
CSB-EP890353DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable methyltransferase PMT23 (At2g40280), partial CSB-YP890354DOA
CSB-EP890354DOA
CSB-BP890354DOA
CSB-MP890354DOA
CSB-EP890354DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Shikimate kinase, chloroplastic (At2g21940) CSB-YP890355DOA
CSB-EP890355DOA
CSB-BP890355DOA
CSB-MP890355DOA
CSB-EP890355DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ribonucleoside-diphosphate reductase large subunit (RNR1), partial CSB-YP890356DOA
CSB-EP890356DOA
CSB-BP890356DOA
CSB-MP890356DOA
CSB-EP890356DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana BEL1-like homeodomain protein 1 (BLH1), partial CSB-YP890357DOA
CSB-EP890357DOA
CSB-BP890357DOA
CSB-MP890357DOA
CSB-EP890357DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana BTB/POZ domain-containing protein At2g04740 (At2g04740), partial CSB-YP890358DOA
CSB-EP890358DOA
CSB-BP890358DOA
CSB-MP890358DOA
CSB-EP890358DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana GDSL esterase/lipase At2g24560 (At2g24560) CSB-YP890359DOA
CSB-EP890359DOA
CSB-BP890359DOA
CSB-MP890359DOA
CSB-EP890359DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Magnesium-chelatase subunit chlD, chloroplastic (CHLD), partial CSB-YP890360DOA
CSB-EP890360DOA
CSB-BP890360DOA
CSB-MP890360DOA
CSB-EP890360DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At2g42920, chloroplastic (PCMP-E75), partial CSB-YP890361DOA
CSB-EP890361DOA
CSB-BP890361DOA
CSB-MP890361DOA
CSB-EP890361DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana RING-H2 finger protein ATL57 (ATL57), partial CSB-YP890362DOA1
CSB-EP890362DOA1
CSB-BP890362DOA1
CSB-MP890362DOA1
CSB-EP890362DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Membrin-11 (MEMB11), partial CSB-YP890363DOA
CSB-EP890363DOA
CSB-BP890363DOA
CSB-MP890363DOA
CSB-EP890363DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uridine nucleosidase 1 (URH1) CSB-YP890364DOA
CSB-EP890364DOA
CSB-BP890364DOA
CSB-MP890364DOA
CSB-EP890364DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative fucosyltransferase 10 (FUT10), partial CSB-YP890365DOA
CSB-EP890365DOA
CSB-BP890365DOA
CSB-MP890365DOA
CSB-EP890365DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Coatomer subunit alpha-2 (At2g21390), partial CSB-YP890366DOA
CSB-EP890366DOA
CSB-BP890366DOA
CSB-MP890366DOA
CSB-EP890366DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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