Bovine Epithelial growth factor(EGF)ELISA Kit

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

牛表皮生長因子(EGF)酶聯(lián)免疫試劑盒（CSB-E17171B）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的EGF含量。EGF（表皮生長因子）是EGF家族的創(chuàng)始成員，具有53個氨基酸殘基和三個分子內二硫鍵。通過與細胞表面的EGFR受體結合，EGF刺激細胞生長、分化和存活，參與多種生理和病理過程，如組織再生、傷口愈合和腫瘤發(fā)生。EGF通過激活EGFR的酪氨酸激酶活性，啟動信號轉導級聯(lián)反應，導致細胞內鈣水平升高、糖酵解和蛋白質合成增加，最終促進DNA合成和細胞增殖。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，該產品可廣泛應用于動物生理學研究、營養(yǎng)代謝評估、疾病模型構建等科研領域，為探究牛EGF在生長發(fā)育調控、免疫應答及病理過程中的分子機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
別名：

HOMG4, URG, beta-urogastrone|epidermal growth factor
縮寫：

EGF
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	88-97
1:2	Average %	106
	Range %	100-109
1:4	Average %	90
	Range %	85-94
1:8	Average %	94
	Range %	90-97

回收率：

The recovery of bovine EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-97
EDTA plasma (n=4)	94	89-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.