Chicken Epidermal growth factor,EGF ELISA Kit

產(chǎn)品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

雞表皮生長因子(EGF)酶聯(lián)免疫試劑盒（CSB-E09868Ch）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的EGF含量。EGF即表皮生長因子，是一種廣泛存在于人和動物體內(nèi)的小分子多肽。其可促進細胞分裂增殖。在研究機制方面，它能與細胞表面的EGF受體結(jié)合，激活細胞內(nèi)信號通路，在組織修復(fù)、腫瘤發(fā)生發(fā)展等研究領(lǐng)域備受關(guān)注。試劑盒檢測范圍為125 pg/mL-8000 pg/mL，適用于家禽生長調(diào)控、組織修復(fù)等基礎(chǔ)研究領(lǐng)域；科研人員可通過動態(tài)監(jiān)測實驗動物EGF分泌水平，用于家禽生理機制研究、營養(yǎng)干預(yù)實驗或疾病模型中的生長因子表達分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

EGF
種屬：

Gallus gallus (Chicken)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

125 pg/mL-8000 pg/mL
靈敏度：

31.25 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	100-104
1:2	Average %	95
	Range %	92-98
1:4	Average %	88
	Range %	85-91
1:8	Average %	99
	Range %	96-102

回收率：

The recovery of chicken EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	102-106
EDTA plasma (n=4)	93	90-96

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.