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Canine Creatine Kinase MB isoenzyme (CK-MB)ELISA Kit

  • 中文名稱:
    犬肌酸激酶同工酶MB(CK-MB)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E15852c
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3800/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    犬肌酸激酶同工酶MB(CK-MB)酶聯(lián)免疫試劑盒(CSB-E15852c)為競爭法ELISA試劑盒,定量檢測血清、血漿樣本中的CK-MB含量。CK-MB即肌酸激酶同工酶MB,主要存在于心肌中。在急性心肌梗死等心肌損傷時,其釋放入血,水平會顯著升高。研究機制方面,通過檢測血液中CK-MB活性或含量變化,可輔助診斷心肌損傷情況、判斷病情發(fā)展及評估治療效果。試劑盒檢測范圍為1.56 μIU/mL-100 μIU/mL,適用于犬類模型的心肌損傷相關科研實驗,例如評估心肌缺血再灌注損傷程度、監(jiān)測心臟毒性藥物對心肌的影響或探究心臟疾病模型的病理進程。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫:
    CK-MB
  • 種屬:
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 樣本類型:
    serum, plasma
  • 檢測范圍:
    1.56 μIU/mL-100 μIU/mL
  • 靈敏度:
    0.78 μIU/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cardiovascular
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of canine CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:100 Average % 84
    Range % 80-88
    1:200 Average % 98
    Range % 94-102
    1:400 Average % 90
    Range % 86-94
    1:800 Average % 92
    Range % 88-96
  • 回收率:
    The recovery of canine CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 100 95-107
    EDTA plasma (n=4) 89 85-93
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μIU/ml OD1 OD2 Average
    100 0.157 0.162 0.160
    50 0.301 0.284 0.293
    25 0.464 0.491 0.478
    12.5 0.736 0.715 0.726
    6.25 0.982 0.959 0.971
    3.12 1.275 1.307 1.291
    1.56 1.513 1.563 1.538
    0 2.029 2.126 2.078
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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