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  4. Mouse Creatine Kinase MB isoenzyme,CK-MB ELISA Kit

Mouse Creatine Kinase MB isoenzyme,CK-MB ELISA Kit

  • 中文名稱：
    小鼠肌酸激酶同工酶MB(CK-MB)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E14404m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠肌酸激酶同工酶MB(CK-MB)酶聯(lián)免疫試劑盒（CSB-E14404m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的CK-MB含量。CK-MB即肌酸激酶同工酶，是心肌損傷的重要標(biāo)志物。其釋放量與心肌損傷程度相關(guān)。在研究機(jī)制上，主要通過檢測血液中CK-MB水平變化，輔助診斷急性心肌梗死等心臟疾病，評估病情及預(yù)后。試劑盒檢測范圍為1.25 mU/mL-80 mU/mL，適用于心血管疾病機(jī)制研究、心肌缺血再灌注損傷模型評估、藥物心臟毒性篩選等科研場景本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    CK-MB
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    1.25 mU/mL-80 mU/mL
  • 靈敏度：
    0.312 mU/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 80-99  
    1:2 Average % 95  
    Range % 86-105  
    1:4 Average % 91  
    Range % 82-100  
    1:8 Average % 93  
    Range % 82-101  
  • 回收率：
    The recovery of mouse CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 94-105  
    EDTA plasma (n=4) 95 89-98  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/ml OD1 OD2 Average Corrected  
    80 2.912 2.787 2.850 2.684  
    40 2.386 2.374 2.380 2.214  
    20 1.617 1.664 1.641 1.475  
    10 0.846 0.895 0.871 0.705  
    5 0.553 0.523 0.538 0.372  
    2.5 0.318 0.336 0.327 0.161  
    1.25 0.246 0.252 0.249 0.083  
    0 0.164 0.168 0.166 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價(jià)

平均分:
5.0分 - 1 個(gè)評價(jià)

樣品類型：血清

樣品信息：小鼠

稀釋比：沒有稀釋

產(chǎn)品評價(jià)： 我用CSB-E14404m檢測小鼠血清中CK-MB含量，未進(jìn)行稀釋，結(jié)果在標(biāo)曲范圍內(nèi)，靈敏度優(yōu)異，標(biāo)曲的OD值分別為0.117，0.13，0.148，0.185，0.222，0.619，1.472，2.755

By 沈老師