Pig creatine kinase MB isoenzyme (CK-MB) ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

豬肌酸激酶同工酶MB(CK-MB)酶聯(lián)免疫試劑盒（CSB-EQ027653PI）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CK-MB含量。CK - MB即肌酸激酶同工酶，主要存在于心肌中。其水平變化可反映心肌損傷情況，是診斷心肌梗死等疾病的重要指標(biāo)。研究機(jī)制上，可通過檢測(cè)血液中其含量變化，輔助判斷病情嚴(yán)重程度、評(píng)估治療效果等。試劑盒檢測(cè)范圍為2.35 mU/mL-150 mU/mL，適用于豬心血管疾病基礎(chǔ)研究、心肌損傷模型驗(yàn)證、藥物心臟毒性評(píng)估及疾病機(jī)制探索等科研領(lǐng)域。研究人員可通過高效分析不同處理?xiàng)l件下心肌損傷的動(dòng)態(tài)變化，為動(dòng)物模型研究及生物醫(yī)學(xué)實(shí)驗(yàn)提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

CK-MB
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

2.35 mU/mL-150 mU/mL
靈敏度：

0.58 mU/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cardiovascular
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	86-98
1:2	Average %	95
	Range %	89-104
1:4	Average %	95
	Range %	87-102
1:8	Average %	95
	Range %	88-100

回收率：

The recovery of pig CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	92-105
EDTA plasma (n=4)	92	88-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.