Human Creatine Kinase MB isoenzyme,CK-MB ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

人肌酸激酶同工酶MB(CK-MB)酶聯(lián)免疫試劑盒（CSB-E05140h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、尿液、組織勻漿樣本中的CK-MB含量。CK-MB即肌酸激酶同工酶，主要存在于心肌中。它是診斷急性心肌梗死等心臟疾病的重要生物標志物。研究機制是當心肌受損時，CK-MB會釋放進入血液，通過檢測血中其含量變化，可輔助判斷心肌損傷程度、范圍及病情發(fā)展。試劑盒檢測范圍為0.9 ng/mL-60 ng/mL，本試劑盒適用于科研領(lǐng)域中心肌損傷相關(guān)疾病模型的機制探索，如心肌梗死、心肌炎或藥物心臟毒性評估等研究場景，可通過檢測不同樣本類型（如血液樣本動態(tài)監(jiān)測病程進展，尿液樣本探索新型標志物潛力，或組織勻漿分析離體心肌損傷）為心血管病理研究提供定量數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

CK-MB
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, urine, tissue homogenates
檢測范圍：

0.9 ng/mL-60 ng/mL
靈敏度：

0.22 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	84-90
1:2	Average %	104
	Range %	101-107
1:4	Average %	98
	Range %	94-102
1:8	Average %	101
	Range %	98-104

回收率：

The recovery of human CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-89
EDTA plasma (n=4)	109	104-113

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.