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  4. Fish dopamine(DA) ELISA kit

Fish dopamine(DA) ELISA kit

  • 中文名稱：
    魚多巴胺(DA)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EQ027496FI
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    魚多巴胺(DA)酶聯(lián)免疫試劑盒（CSB-EQ027496FI）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的DA含量。DA即多巴胺，是腦內(nèi)重要神經(jīng)遞質(zhì)，在運(yùn)動(dòng)控制、認(rèn)知、情感及內(nèi)分泌調(diào)節(jié)等過程中發(fā)揮關(guān)鍵作用。研究機(jī)制方面，圍繞其合成、釋放、與受體結(jié)合及代謝等環(huán)節(jié)展開，探究其在帕金森病、精神分裂癥等疾病中異常變化以尋找治療靶點(diǎn)。試劑盒檢測(cè)范圍為62.5 pg/mL-1000 pg/mL，該產(chǎn)品適用于魚類神經(jīng)生物學(xué)研究、水產(chǎn)養(yǎng)殖健康監(jiān)測(cè)、環(huán)境污染物對(duì)水生生物神經(jīng)系統(tǒng)的毒性評(píng)估等科研領(lǐng)域，特別在水體環(huán)境毒理學(xué)實(shí)驗(yàn)中，可通過檢測(cè)魚類血液或組織DA水平評(píng)估重金屬、農(nóng)藥等污染物對(duì)中樞神經(jīng)系統(tǒng)的影響。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    DA
  • 種屬：
    Fish
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    62.5 pg/mL-1000 pg/mL
  • 靈敏度：
    62.5 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish DA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 92-104  
    1:2 Average % 94  
    Range % 91-98  
    1:4 Average % 102  
    Range % 98-106  
    1:8 Average % 88  
    Range % 82-94  
  • 回收率：
    The recovery of fish DA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-98  
    EDTA plasma (n=4) 103 98-107  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    1000 0.154 0.155 0.155    
    500 0.278 0.272 0.275    
    250 0.503 0.518 0.511    
    125 1.135 1.174 1.155    
    62.5 2.345 2.428 2.387    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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