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  4. Mouse dopamine,DA ELISA Kit

Mouse dopamine,DA ELISA Kit

  • 中文名稱：
    小鼠多巴胺(DA)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08661m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠多巴胺(DA)酶聯(lián)免疫試劑盒（CSB-E08661m）為競(jìng)爭法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿.樣本中的DA含量。DA即多巴胺，是重要神經(jīng)遞質(zhì)。其參與運(yùn)動(dòng)控制、情緒調(diào)節(jié)等生理過程。研究發(fā)現(xiàn)DA信號(hào)異常與帕金森病、精神分裂癥等疾病有關(guān)。當(dāng)前研究圍繞DA受體亞型、轉(zhuǎn)運(yùn)體等機(jī)制，旨在開發(fā)針對(duì)性藥物治療神經(jīng)及精神類疾病。試劑盒檢測(cè)范圍為15.6 pg/ml-1000 pg/ml.，科研人員可將其應(yīng)用于神經(jīng)生物學(xué)基礎(chǔ)研究，如評(píng)估腦區(qū)組織勻漿中DA的動(dòng)態(tài)變化，或探究疾病模型中血漿DA水平的調(diào)控機(jī)制；也可用于藥理學(xué)實(shí)驗(yàn)，分析藥物干預(yù)對(duì)多巴胺能系統(tǒng)的影響，為神經(jīng)疾病機(jī)制解析及潛在治療策略開發(fā)提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    N/A
  • 縮寫：
    DA
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates.
  • 檢測(cè)范圍：
    15.6 pg/ml-1000 pg/ml.
  • 靈敏度：
    3.9pg/ml.
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse dopamine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    107

    Range %

    103-112

    1:2

    Average %

    95

    Range %

    91-105

    1:4

    Average %

    105

    Range %

    100-111

    1:8

    Average %

    93

    Range %

    86-98

  • 回收率：

    The recovery of mouse dopamine spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    89-98

    EDTA plasma (n=4)

    93

    88-100

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    1000

    0.151

    0.144

    0.148

    500

    0.224

    0.217

    0.221

    250

    0.341

    0.328

    0.335

    125

    0.488

    0.519

    0.503

    62.5

    0.699

    0.721

    0.710

    31.25

    1.018

    1.053

    1.035

    15.6

    1.468

    1.384

    1.426

    0

    2.417

    2.325

    2.371

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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