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  4. Human dopamine,DA ELISA KIT

Human dopamine,DA ELISA KIT

  • 中文名稱：
    人多巴胺(DA)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E06888h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人多巴胺(DA)酶聯(lián)免疫試劑盒（CSB-E06888h）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的DA含量。DA即多巴胺，是一種重要神經(jīng)遞質(zhì)。其在調(diào)節(jié)運動、情感、認(rèn)知等生理過程中發(fā)揮關(guān)鍵作用，異常時會引發(fā)如帕金森病、精神分裂癥等多種疾病。相關(guān)研究聚焦于其合成、釋放、代謝機制及與受體相互作用，為開發(fā)針對性治療藥物提供方向。試劑盒檢測范圍為0.5 ng/mL-100 ng/mL，該產(chǎn)品支持多樣本類型分析，便于研究人員探索不同生物基質(zhì)中多巴胺的動態(tài)變化，如神經(jīng)藥理學(xué)研究中藥物干預(yù)對神經(jīng)遞質(zhì)釋放的影響、疾病模型動物腦組織多巴胺代謝評估，或體外培養(yǎng)神經(jīng)元/神經(jīng)母細(xì)胞瘤細(xì)胞的多巴胺分泌水平監(jiān)測。適用于神經(jīng)生物學(xué)、行為學(xué)及分子機制研究中的多巴胺定量分析需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    N/A
  • 縮寫：
    DA
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    0.5 ng/mL-100 ng/mL
  • 靈敏度：
    0.25 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human DA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 90-98  
    1:2 Average % 90  
    Range % 86-94  
    1:4 Average % 90  
    Range % 86-94  
    1:8 Average % 102  
    Range % 97-105  
  • 回收率：
    The recovery of human DA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 91-98  
    EDTA plasma (n=4) 94 89-98  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    100 0.220 0.216 0.218    
    25 0.328 0.337 0.333    
    8 0.512 0.532 0.522    
    2 0.884 0.864 0.874    
    0.5 1.230 1.217 1.224    
    0 1.903 2.021 1.962    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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