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Rat dopamine,DA ELISA Kit

  • 中文名稱:
    大鼠多巴胺(DA)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E08660r
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3900/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    大鼠多巴胺(DA)酶聯(lián)免疫試劑盒(CSB-E08660r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的DA含量。DA即多巴胺,是一種重要神經(jīng)遞質(zhì)。其在調(diào)節(jié)運動、情緒、獎賞等生理功能中有關(guān)鍵作用。研究機制上,圍繞其合成、釋放、再攝取等過程,探究與帕金森病、精神分裂癥等疾病聯(lián)系,以開發(fā)針對性治療藥物。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL,該產(chǎn)品適用于神經(jīng)生物學、藥理學等科研領(lǐng)域,可探究帕金森模型動物DA水平變化、精神疾病相關(guān)神經(jīng)遞質(zhì)動態(tài),或評估藥物干預(yù)對DA代謝通路的影響。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    N/A
  • 縮寫:
    DA
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.156 ng/mL-10 ng/mL
  • 靈敏度:
    0.039 ng/mL
  • 反應(yīng)時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Neuroscience
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat DA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:5 Average % 86  
    Range % 84-96  
    1:10 Average % 95  
    Range % 91-100  
    1:20 Average % 84  
    Range % 81-88  
    1:40 Average % 92  
    Range % 88-96  
  • 回收率:
    The recovery of rat DA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 87-95  
    EDTA plasma (n=4) 96 94-98  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    10 2.174 2.280 2.227 2.076  
    5 1.784 1.789 1.787 1.636  
    2.5 1.250 1.276 1.263 1.112  
    1.25 0.841 0.875 0.858 0.707  
    0.625 0.435 0.465 0.450 0.299  
    0.312 0.287 0.308 0.298 0.147  
    0.156 0.208 0.223 0.216 0.065  
    0 0.152 0.150 0.151    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻

產(chǎn)品評價

靶點詳情

  • 最新研究進展:
    DA是多巴胺的簡稱,是一種重要的神經(jīng)遞質(zhì)。近年來,研究人員發(fā)現(xiàn)DA參與了多種生理和病理過程,如運動功能、認知能力、代謝性疾病、精神疾病等。同時,DA與多種神經(jīng)系統(tǒng)疾病的發(fā)病機制密切相關(guān),如帕金森病、精神分裂癥等。