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  4. Goat Luteinizing Hormone (LH) ELISA KIT

Goat Luteinizing Hormone (LH) ELISA KIT

  • 中文名稱：
    山羊黃體生成素(LH)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13274G
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    山羊黃體生成素(LH)酶聯(lián)免疫試劑盒（CSB-E13274G）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的LH含量。LH即促黃體生成素，是腺垂體分泌的促性腺激素。其在調(diào)節(jié)性腺功能方面起關(guān)鍵作用，可刺激卵巢排卵和黃體形成，促進(jìn)雄激素合成及精子生成。目前對(duì)其分泌調(diào)控、與相關(guān)疾病關(guān)系等機(jī)制是研究熱點(diǎn)，以助解決生殖等方面問(wèn)題。試劑盒檢測(cè)范圍為0.3 mLU/mL-60 mLU/mL，適用于山羊繁殖生理機(jī)制研究、卵巢功能評(píng)估、生殖內(nèi)分泌調(diào)控實(shí)驗(yàn)以及繁殖障礙模型的建立等科研場(chǎng)景，例如通過(guò)動(dòng)態(tài)監(jiān)測(cè)發(fā)情周期中LH濃度波動(dòng)，探究排卵誘導(dǎo)機(jī)制或評(píng)估激素干預(yù)效果本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě)：
    LH
  • 種屬：
    Capra hircus (Goat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.3 mLU/mL-60 mLU/mL
  • 靈敏度：
    0.15 mIU/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of goat LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 98  
    Range % 90-104  
    1:2 Average % 95  
    Range % 90-103  
    1:4 Average % 91  
    Range % 85-96  
    1:8 Average % 90  
    Range % 88-93  
  • 回收率：
    The recovery of goat LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 90-101  
    EDTA plasma (n=4) 92 87-96  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/ml OD1 OD2 Average    
    60 0.149 0.144 0.147    
    15 0.289 0.298 0.294    
    4.8 0.520 0.527 0.524    
    1.2 0.861 0.865 0.863    
    0.3 1.411 1.416 1.414    
    0 2.213 2.212 2.213    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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