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  4. Human oxidized low density lipoprotein,OxLDL ELISA Kit

Human oxidized low density lipoprotein,OxLDL ELISA Kit

  • 中文名稱：
    人氧化低密度脂蛋白(OxLDL)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07931h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人氧化低密度脂蛋白(OxLDL)酶聯(lián)免疫試劑盒（CSB-E07931h）為競爭法ELISA試劑盒，定量檢測血清、血漿、尿液、唾液、組織勻漿、細胞裂解物樣本中的OxLDL含量。OxLDL即氧化型低密度脂蛋白，是一種被氧化修飾的脂蛋白。它與動脈粥樣硬化等疾病密切相關。其研究機制主要聚焦在它可被巨噬細胞攝取形成泡沫細胞，還能引發(fā)炎癥反應，損傷血管內(nèi)皮細胞，促進動脈粥樣硬化斑塊形成。試劑盒檢測范圍為1.56 mU/mL-100 mU/mL，本試劑盒可應用于心血管疾病基礎研究、氧化應激相關代謝機制探索、藥物或天然產(chǎn)物抗氧化效價評估等科研領域，為體外實驗提供高靈敏度的檢測方案，但不適用于臨床診斷。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    OxLDL
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, urine, saliva, tissue homogenates, cell lysates
  • 檢測范圍：
    1.56 mU/mL-100 mU/mL
  • 靈敏度：
    0.78 mU/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human OxLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 99  
    Range % 93-105  
    1:200 Average % 94  
    Range % 91-98  
    1:400 Average % 103  
    Range % 99-107  
    1:800 Average % 86  
    Range % 80-92  
  • 回收率：
    The recovery of human OxLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 94-104  
    EDTA plasma (n=4) 94 88-97  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/ml OD1 OD2 Average    
    100 0.130 0.129 0.130    
    50 0.306 0.301 0.304    
    25 0.549 0.557 0.553    
    12.5 0.941 0.956 0.949    
    6.25 1.401 1.418 1.410    
    3.12 1.953 1.967 1.960    
    1.56 2.186 2.087 2.137    
    0 2.758 2.656 2.707    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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